PMID- 25461142
OWN - NLM
STAT- MEDLINE
DCOM- 20170123
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 65
DP  - 2015 Mar 15
TI  - Onsite naked eye determination of cysteine and homocysteine using quencher 
      displacement-induced fluorescence recovery of the dual-emission hybrid probes 
      with desired intensity ratio.
PG  - 83-90
LID - S0956-5663(14)00780-5 [pii]
LID - 10.1016/j.bios.2014.09.093 [doi]
AB  - Simple, inexpensive, portable sensing strategies for those clinically relevant 
      molecules have attained a significant positive impact on the health care system. 
      Herein, we have prepared a dual-emission ratiometric fluorescence probe with 
      desired intensity ratio and demonstrated its efficiency for onsite naked eye 
      determination of cysteine (Cys) and homocysteine (Hcy). The hybrid probe has been 
      designed by hybridizing two differently sized CdTe quantum dots (QDs), in which 
      the red-emitting CdTe QDs (rQDs) entrapped in the silica sphere acting as the 
      reference signal, and the green-emitting CdTe QDs (gQDs) covalently attached on 
      the silica surface serving as the response signal. When 1,10-phenanthroline with 
      strong coordination ability to Cd atoms in gQDs was introduced, the fluorescence 
      of the gQDs was effectively quenched, while the fluorescence of the rQDs stayed 
      constant. Upon exposure to different contents of Cys or Hcy, the fluorescence of 
      gQDs can be recovered gradually due to the displacement of the quencher. Based on 
      the background signal of rQDs, the variations of the sensing system display 
      continuous fluorescence color changes from red to green, which can be easily 
      observed by the naked eye. The assay requires  approximately 20min and has a detection limit of 
      2.5 and 1.7muM for Cys and Hcy, respectively. Furthermore, we demonstrate that 
      this sensing scheme can be fully integrated in a filter paper-based assay, thus 
      enabling a potential point-of-care application featuring easy operation, low 
      power consumption, and low fabrication costs.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Wang, Kan
AU  - Wang K
AD  - Key Laboratory of Modern Agriculture Equipment and Technology, School of 
      Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR 
      China.
FAU - Qian, Jing
AU  - Qian J
AD  - Key Laboratory of Modern Agriculture Equipment and Technology, School of 
      Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR 
      China.
FAU - Jiang, Ding
AU  - Jiang D
AD  - Key Laboratory of Modern Agriculture Equipment and Technology, School of 
      Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR 
      China.
FAU - Yang, Zhengting
AU  - Yang Z
AD  - Key Laboratory of Modern Agriculture Equipment and Technology, School of 
      Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR 
      China.
FAU - Du, Xiaojiao
AU  - Du X
AD  - Key Laboratory of Modern Agriculture Equipment and Technology, School of 
      Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR 
      China.
FAU - Wang, Kun
AU  - Wang K
AD  - Key Laboratory of Modern Agriculture Equipment and Technology, School of 
      Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR 
      China. Electronic address: wangkun@ujs.edu.cn.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20141005
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Cadmium Compounds)
RN  - 0 (Fluorescent Dyes)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - K848JZ4886 (Cysteine)
RN  - NQA0O090ZJ (Tellurium)
RN  - STG188WO13 (cadmium telluride)
SB  - IM
MH  - Biosensing Techniques/methods
MH  - Cadmium Compounds/*chemistry
MH  - Cysteine/*blood
MH  - Fluorescent Dyes/*chemistry
MH  - Homocysteine/*blood
MH  - Humans
MH  - Limit of Detection
MH  - Point-of-Care Systems
MH  - Quantum Dots/*chemistry
MH  - Spectrometry, Fluorescence/methods
MH  - Tellurium/*chemistry
OTO - NOTNLM
OT  - Cysteine
OT  - Dual-emission hybrid probes
OT  - Homocysteine
OT  - Naked eye determination
OT  - Ratiometric fluorescence
EDAT- 2014/12/03 06:00
MHDA- 2017/01/24 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/07/24 00:00 [received]
PHST- 2014/09/29 00:00 [revised]
PHST- 2014/09/30 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2017/01/24 06:00 [medline]
AID - S0956-5663(14)00780-5 [pii]
AID - 10.1016/j.bios.2014.09.093 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2015 Mar 15;65:83-90. doi: 10.1016/j.bios.2014.09.093. Epub 
      2014 Oct 5.