PMID- 25461451
OWN - NLM
STAT- MEDLINE
DCOM- 20150721
LR  - 20181113
IS  - 1879-0380 (Electronic)
IS  - 0959-437X (Print)
IS  - 0959-437X (Linking)
VI  - 28
DP  - 2014 Oct
TI  - Reprogramming by lineage specifiers: blurring the lines between pluripotency and 
      differentiation.
PG  - 57-63
LID - S0959-437X(14)00104-X [pii]
LID - 10.1016/j.gde.2014.09.009 [doi]
AB  - The generation of human induced pluripotent stem cells (iPS) has raised enormous 
      expectations within the biomedical community due to their potential vast 
      implications in regenerative and personalized medicine. However, reprogramming to 
      iPS is still not fully comprehended. Difficulties found in ascribing specific 
      molecular patterns to pluripotent cells (PSCs), and inherent inter-line and 
      intra-line variability between different PSCs need to be resolved. Additionally, 
      and despite multiple assumptions, it remains unclear whether the current in vitro 
      culturing conditions for the maintenance and differentiation of PSCs do indeed 
      recapitulate the developmental processes observed in vivo. As a consequence, 
      basic questions such as what is the actual nature of PSCs remain unanswered and 
      different theories have emerged in regards to the identity of these valuable cell 
      population. Here we discuss on the published theories for defining PSC identity, 
      the implications that the different postulated models have for the reprogramming 
      field as well as speculate on potential future directions that might be opened 
      once a precise knowledge on the nature of PSCs is accomplished.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Sancho-Martinez, Ignacio
AU  - Sancho-Martinez I
AD  - Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North 
      Torrey Pines Road, La Jolla, CA 92037, USA.
FAU - Ocampo, Alejandro
AU  - Ocampo A
AD  - Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North 
      Torrey Pines Road, La Jolla, CA 92037, USA.
FAU - Izpisua Belmonte, Juan Carlos
AU  - Izpisua Belmonte JC
AD  - Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North 
      Torrey Pines Road, La Jolla, CA 92037, USA. Electronic address: 
      belmonte@salk.edu.
LA  - eng
GR  - U01 HL107442/HL/NHLBI NIH HHS/United States
GR  - 5U01HL107442/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Review
DEP - 20141014
PL  - England
TA  - Curr Opin Genet Dev
JT  - Current opinion in genetics & development
JID - 9111375
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - *Cell Lineage
MH  - *Cellular Reprogramming
MH  - Humans
MH  - Pluripotent Stem Cells/*cytology
PMC - PMC4262641
MID - NIHMS631113
COIS- Competing interests The authors declare no competing financial interests.
EDAT- 2014/12/03 06:00
MHDA- 2015/07/22 06:00
PMCR- 2015/10/14
CRDT- 2014/12/03 06:00
PHST- 2014/07/09 00:00 [received]
PHST- 2014/08/24 00:00 [revised]
PHST- 2014/09/16 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/07/22 06:00 [medline]
PHST- 2015/10/14 00:00 [pmc-release]
AID - S0959-437X(14)00104-X [pii]
AID - 10.1016/j.gde.2014.09.009 [doi]
PST - ppublish
SO  - Curr Opin Genet Dev. 2014 Oct;28:57-63. doi: 10.1016/j.gde.2014.09.009. Epub 2014 
      Oct 14.