PMID- 25462438
OWN - NLM
STAT- MEDLINE
DCOM- 20150804
LR  - 20181113
IS  - 1879-6265 (Electronic)
IS  - 1879-6257 (Print)
IS  - 1879-6257 (Linking)
VI  - 9
DP  - 2014 Dec
TI  - Past, present, and future perspectives on the diagnosis of Roseolovirus 
      infections.
PG  - 84-90
LID - S1879-6257(14)00194-1 [pii]
LID - 10.1016/j.coviro.2014.09.014 [doi]
AB  - Diagnosis of Roseolovirus infections mandates careful selection of patients, 
      samples, and testing methods. We review advances in the field and highlight 
      research priorities. Quantitative (q)PCR can accurately identify and distinguish 
      between human herpesvirus 6 (HHV-6) species A and B. Whether screening of 
      high-risk patients improves outcomes is unclear. Chromosomally integrated 
      (ci)HHV-6 confounds test interpretation but can be ruled out with digital PCR. 
      Reverse transcription qPCR may be a more specific and clinically applicable test 
      for actively replicating Roseoloviruses, particularly among patients with 
      ciHHV-6. Interpretation of Roseolovirus test results faces many challenges. 
      However, careful application of refined and emerging diagnostic techniques will 
      allow for increasingly accurate diagnosis of clinically significant infections 
      and disease associations.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Hill, Joshua A
AU  - Hill JA
AD  - Department of Medicine, University of Washington, Seattle, WA, United States; 
      Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, 
      Seattle, WA, United States.
FAU - Sedlak, Ruth Hall
AU  - Sedlak RH
AD  - Department of Laboratory Medicine, University of Washington, Seattle, WA, United 
      States.
FAU - Jerome, Keith R
AU  - Jerome KR
AD  - Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, 
      Seattle, WA, United States; Department of Laboratory Medicine, University of 
      Washington, Seattle, WA, United States. Electronic address: 
      kjerome@u.washington.edu.
LA  - eng
GR  - T32 AI007044/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Review
DEP - 20141014
PL  - Netherlands
TA  - Curr Opin Virol
JT  - Current opinion in virology
JID - 101560941
SB  - IM
MH  - Diagnostic Tests, Routine/*methods/trends
MH  - Humans
MH  - Molecular Diagnostic Techniques/*methods/trends
MH  - Real-Time Polymerase Chain Reaction/methods
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Roseolovirus/genetics/*isolation & purification
MH  - Roseolovirus Infections/*diagnosis
MH  - Virology/*methods/trends
PMC - PMC4573551
MID - NIHMS721612
EDAT- 2014/12/03 06:00
MHDA- 2015/08/05 06:00
PMCR- 2015/09/17
CRDT- 2014/12/03 06:00
PHST- 2014/08/14 00:00 [received]
PHST- 2014/09/22 00:00 [revised]
PHST- 2014/09/22 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/08/05 06:00 [medline]
PHST- 2015/09/17 00:00 [pmc-release]
AID - S1879-6257(14)00194-1 [pii]
AID - 10.1016/j.coviro.2014.09.014 [doi]
PST - ppublish
SO  - Curr Opin Virol. 2014 Dec;9:84-90. doi: 10.1016/j.coviro.2014.09.014. Epub 2014 
      Oct 14.