PMID- 25463865
OWN - NLM
STAT- MEDLINE
DCOM- 20150527
LR  - 20220330
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 112
DP  - 2015 Feb
TI  - Combined toxicity of amorphous silica nanoparticles and methylmercury to human 
      lung epithelial cells.
PG  - 144-52
LID - S0147-6513(14)00492-8 [pii]
LID - 10.1016/j.ecoenv.2014.10.026 [doi]
AB  - Exposure to the ambient particulate matters (PM) has been associated with the 
      morbidity and mortality of cardiopulmonary diseases. Compared with coarse 
      particles, ultrafine particles (UFP) absorb or condense higher concentration of 
      toxic air pollutants and are easily inhaled into the lung. However, the combined 
      effects of UFP and air pollutants on human health are still poorly understood. In 
      this study, a co-exposure in vitro model of amorphous silica nanoparticles 
      (nano-SiO2) and methyl mercury (MeHg) was established to investigate their 
      combined effects and the potential joint action type. Lung adenocarcinoma cells 
      (A549) were exposed to either nano-SiO2 or MeHg alone, or a combination of both. 
      Factorial design was applied to analyze their potential joint action type. Higher 
      interfacial energy was observed in the mixed solution of nano-SiO2 and MeHg. The 
      intracellular content of both silicon and mercury in combination group were much 
      higher than those in single exposure groups. In addition, the co-exposure of 
      nano-SiO2 and MeHg enhanced the reactive oxygen species (ROS) generation, lipid 
      peroxidation and reduced the activity of superoxide dismutase (SOD) and 
      glutathione peroxidase (GSH-px). The excessive oxidative stress led to oxidative 
      DNA damage as well as cellular apoptosis. Factorial design analysis demonstrated 
      that additive and synergistic interactions were responsible for the combined 
      toxicity of nano-SiO2 and MeHg.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Yu, Yongbo
AU  - Yu Y
AD  - School of Public Health, Capital Medical University, Beijing 100069, PR China; 
      Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, 
      Beijing 100069, PR China.
FAU - Duan, Junchao
AU  - Duan J
AD  - School of Public Health, Capital Medical University, Beijing 100069, PR China; 
      Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, 
      Beijing 100069, PR China.
FAU - Li, Yang
AU  - Li Y
AD  - School of Public Health, Capital Medical University, Beijing 100069, PR China; 
      Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, 
      Beijing 100069, PR China.
FAU - Yu, Yang
AU  - Yu Y
AD  - School of Public Health, Capital Medical University, Beijing 100069, PR China; 
      Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, 
      Beijing 100069, PR China.
FAU - Jin, Minghua
AU  - Jin M
AD  - School of Public Health, Jilin University, Changchun, Jilin 130021, PR China.
FAU - Li, Chenxu
AU  - Li C
AD  - School of Public Health, Jilin University, Changchun, Jilin 130021, PR China.
FAU - Wang, Yapei
AU  - Wang Y
AD  - Department of Chemistry, Renmin University of China, Beijing 100872, PR China. 
      Electronic address: yapeiwang@ruc.edu.cn.
FAU - Sun, Zhiwei
AU  - Sun Z
AD  - School of Public Health, Capital Medical University, Beijing 100069, PR China; 
      Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, 
      Beijing 100069, PR China. Electronic address: zwsun@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141115
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Air Pollutants)
RN  - 0 (Methylmercury Compounds)
RN  - 0 (Particulate Matter)
RN  - 0 (Reactive Oxygen Species)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - RWZ4L3O1X0 (methylmercuric chloride)
SB  - IM
MH  - Air Pollutants/*toxicity
MH  - Cell Line, Tumor
MH  - DNA Damage
MH  - Epithelial Cells/drug effects
MH  - Humans
MH  - Lung/drug effects
MH  - Methylmercury Compounds/*toxicity
MH  - Nanoparticles/*toxicity
MH  - Oxidative Stress/*drug effects
MH  - Particulate Matter/*toxicity
MH  - Reactive Oxygen Species/metabolism
MH  - Silicon Dioxide/*toxicity
OTO - NOTNLM
OT  - Combined toxicity
OT  - Interaction
OT  - Methyl mercury
OT  - Silica nanoparticles
OT  - Ultrafine particles (UFP)
EDAT- 2014/12/03 06:00
MHDA- 2015/05/28 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/08/06 00:00 [received]
PHST- 2014/10/20 00:00 [revised]
PHST- 2014/10/21 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/05/28 06:00 [medline]
AID - S0147-6513(14)00492-8 [pii]
AID - 10.1016/j.ecoenv.2014.10.026 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2015 Feb;112:144-52. doi: 10.1016/j.ecoenv.2014.10.026. 
      Epub 2014 Nov 15.