PMID- 25463904
OWN - NLM
STAT- MEDLINE
DCOM- 20151023
LR  - 20141220
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 60
IP  - 2
DP  - 2015 Feb
TI  - Identification of non-streptococcal organisms from human dental plaque grown on 
      the Streptococcus-selective medium mitis-salivarius agar.
PG  - 267-71
LID - S0003-9969(14)00281-7 [pii]
LID - 10.1016/j.archoralbio.2014.11.002 [doi]
AB  - OBJECTIVE: Mitis-salivarius (MS) agar has been used widely in microbial 
      epidemiological studies because oral viridans streptococci can be selectively 
      grown on this medium. Even though the previous findings reported the limited 
      selecting power of MS agar for streptococcus strains, the identities of 
      non-streptococcal strains from human oral samples which can grow on this medium 
      are not clear yet. In this study, we identified non-streptococcal organisms grown 
      on MS agar plates by polymerase chain reaction (PCR) amplification and sequencing 
      of the 16S ribosomal RNA (rRNA) gene. DESIGN: Eighty bacterial colonies on MS 
      plates were isolated from plaque samples, and bacterial identification was 
      achieved with the rapid ID 32 Strep system and mini API reader. The bacterial 
      colonies identified as non-streptococci by the API system were selected for 
      further identification. The 16S rRNA gene was amplified by PCR and verified using 
      DNA sequencing analysis for identification. Sequences were compared with those of 
      reference organisms in the genome database of the National Center for 
      Biotechnology Information using the Basic Local Alignment Search Tool (BLAST). 
      RESULTS: Among the 11 isolated non-streptococcal strains on MS plates, 3 strains 
      were identified as Actinomyces naeslundii, 7 strains were identified as 
      Actinomyces oris and 1 strain were identified as Actinomyces sp. using Blastn. 
      CONCLUSIONS: In this study, we showed that some oral Actinomyces species can grow 
      on Streptococcus-selective MS agar plates.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Kim, Yeon-Hee
AU  - Kim YH
AD  - Department of Microbiology and Immunology, College of Dentistry, Research 
      Institute of Oral Science, Gangneung-Wonju National University, Gangneung 
      210-702, Republic of Korea.
FAU - Lee, Si Young
AU  - Lee SY
AD  - Department of Microbiology and Immunology, College of Dentistry, Research 
      Institute of Oral Science, Gangneung-Wonju National University, Gangneung 
      210-702, Republic of Korea. Electronic address: siyoung@gwnu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141108
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Culture Media)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Actinomyces/*isolation & purification
MH  - Culture Media
MH  - DNA, Bacterial/analysis
MH  - Dental Plaque/*microbiology
MH  - Female
MH  - Humans
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Streptococcus/*isolation & purification
OTO - NOTNLM
OT  - Actinomyces
OT  - Dental plaque
OT  - Mitis-salivarius agar
OT  - Streptococcus
EDAT- 2014/12/03 06:00
MHDA- 2015/10/24 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/04/23 00:00 [received]
PHST- 2014/09/02 00:00 [revised]
PHST- 2014/11/03 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/10/24 06:00 [medline]
AID - S0003-9969(14)00281-7 [pii]
AID - 10.1016/j.archoralbio.2014.11.002 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2015 Feb;60(2):267-71. doi: 10.1016/j.archoralbio.2014.11.002. 
      Epub 2014 Nov 8.