PMID- 25464018
OWN - NLM
STAT- MEDLINE
DCOM- 20151008
LR  - 20151119
IS  - 1873-2933 (Electronic)
IS  - 0009-9120 (Linking)
VI  - 48
IP  - 3
DP  - 2015 Feb
TI  - Methodological considerations to determine the effect of exercise on 
      brain-derived neurotrophic factor levels.
PG  - 162-6
LID - S0009-9120(14)00772-3 [pii]
LID - 10.1016/j.clinbiochem.2014.11.013 [doi]
AB  - OBJECTIVES: Physical exercise up-regulates brain-derived neurotrophic factor 
      (BDNF) in the brain and blood. However, there is yet no consensus about the 
      adequate blood processing conditions to standardize its assessment. We aimed to 
      find a reliable blood sample processing method to determine changes in BDNF due 
      to exercise. DESIGN AND METHODS: Twelve healthy university students performed an 
      incremental cycling test to exhaustion. At baseline, immediately after exercise, 
      and 30 and 60 min of recovery, venous blood was drawn and processed under 
      different conditions, i.e. whole blood, serum coagulated for 10 min and 24 h, 
      total plasma, and platelet-free plasma. BDNF concentration was measured by ELISA. 
      RESULTS: Exercise increased BDNF in whole blood and in serum coagulated for 24 h 
      when corrected by hemoconcentration. We did not find effects of exercise on BDNF 
      in serum coagulated for 10 min or in plasma samples. Plasma shows heterogeneous 
      BDNF values in response to exercise that are not prevented when platelets are 
      eliminated while homogeneous BDNF levels were found in whole blood or serum 
      coagulated for 24 hour samples. CONCLUSIONS: In exercise studies, BDNF levels 
      should be adjusted by hemoconcentration. Our data highlight the importance of 
      blood sample selection since the differences between each one affect 
      significantly the BDNF factor changes due to exercise.
CI  - Copyright (c) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier 
      Inc. All rights reserved.
FAU - Pareja-Galeano, Helios
AU  - Pareja-Galeano H
AD  - Department of Physiology, School of Medicine, University of Valencia, INCLIVA, 
      Valencia, Spain. Electronic address: helios.pareja@gmail.com.
FAU - Alis, Rafael
AU  - Alis R
AD  - School of Medicine and Research Institute "Dr. Vina Giner", Molecular and 
      Mitochondrial Medicine, Catholic University of Valencia San Vicente Martir, 
      Valencia, Spain.
FAU - Sanchis-Gomar, Fabian
AU  - Sanchis-Gomar F
AD  - Department of Physiology, School of Medicine, University of Valencia, INCLIVA, 
      Valencia, Spain.
FAU - Cabo, Helena
AU  - Cabo H
AD  - Department of Physiology, School of Medicine, University of Valencia, INCLIVA, 
      Valencia, Spain.
FAU - Cortell-Ballester, Jose
AU  - Cortell-Ballester J
AD  - Hospital La Fe., Valencia, Spain.
FAU - Gomez-Cabrera, Mari Carmen
AU  - Gomez-Cabrera MC
AD  - Department of Physiology, School of Medicine, University of Valencia, INCLIVA, 
      Valencia, Spain.
FAU - Lucia, Alejandro
AU  - Lucia A
AD  - European University, Madrid, Spain; Research Institute of Hospital 12 de Octubre 
      ('i+12'), Madrid, Spain.
FAU - Vina, Jose
AU  - Vina J
AD  - Department of Physiology, School of Medicine, University of Valencia, INCLIVA, 
      Valencia, Spain.
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141121
PL  - United States
TA  - Clin Biochem
JT  - Clinical biochemistry
JID - 0133660
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Adult
MH  - Blood Specimen Collection/*methods
MH  - Brain-Derived Neurotrophic Factor/*blood
MH  - Energy Metabolism
MH  - Exercise/*physiology
MH  - Humans
MH  - Male
MH  - Serum/metabolism
MH  - Surveys and Questionnaires
OTO - NOTNLM
OT  - Enzyme-linked immunosorbent assay
OT  - Hemoconcentration
OT  - Plasma
OT  - Serum
OT  - Whole blood
EDAT- 2014/12/03 06:00
MHDA- 2015/10/09 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/10/02 00:00 [received]
PHST- 2014/11/09 00:00 [revised]
PHST- 2014/11/11 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/10/09 06:00 [medline]
AID - S0009-9120(14)00772-3 [pii]
AID - 10.1016/j.clinbiochem.2014.11.013 [doi]
PST - ppublish
SO  - Clin Biochem. 2015 Feb;48(3):162-6. doi: 10.1016/j.clinbiochem.2014.11.013. Epub 
      2014 Nov 21.