PMID- 25465662
OWN - NLM
STAT- MEDLINE
DCOM- 20160502
LR  - 20181202
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 174
IP  - 3-4
DP  - 2014 Dec 5
TI  - In vivo effect of deoxynivalenol (DON) naturally contaminated feed on porcine 
      reproductive and respiratory syndrome virus (PRRSV) infection.
PG  - 419-426
LID - S0378-1135(14)00497-0 [pii]
LID - 10.1016/j.vetmic.2014.10.019 [doi]
AB  - Deoxynivalenol (DON), also known as vomitoxin, is the most prevalent type B 
      trichothecene mycotoxin worldwide. Pigs show a great sensitivity to DON, and 
      because of the high proportion of grains in their diets, they are frequently 
      exposed to this mycotoxin. The objective of this study was to determine the 
      impact of DON naturally contaminated feed on porcine reproductive and respiratory 
      syndrome virus (PRRSV) infection, the most important porcine viral pathogen in 
      swine. Experimental infections were performed with 30 animals. Piglets were 
      randomly divided into three groups of 10 animals based on DON content of diets 
      (0, 2.5 and 3.5 mg/kg DON). All experimental groups were further divided into 
      subgroups of 6 pigs and were inoculated with PRRSV. The remaining pigs (control) 
      were sham-inoculated with PBS. Pigs were daily monitored for temperature, weight 
      and clinical signs for 21 days. Blood samples were collected and tested for PRRSV 
      RNA and for virus specific antibodies. Results of PRRSV infection showed that 
      ingestion of diet highly contaminated with DON greatly increases the effect of 
      PRRSV infection on weight gain, lung lesions and mortality, without increasing 
      significantly viral replication, for which the tendency is rather directed toward 
      a decrease of replication. These results suggest that PRRSV infection could 
      exacerbate anorectic effect of DON, when ingested in large doses. Results also 
      demonstrate a DON negative effect on PRRSV-specific humoral responses. This study 
      demonstrate that high concentrations of DON naturally contaminated feed decreased 
      the immune response against PRRSV and influenced the course of PRRSV infection in 
      pigs.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Savard, Christian
AU  - Savard C
AD  - Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Swine and 
      Poultry Infectious Diseases Research Center (CRIPA), Saint-Hyacinthe, Quebec, 
      Canada.
FAU - Pinilla, Vicente
AU  - Pinilla V
AD  - Departement de Biomedecine Veterinaire, Saint-Hyacinthe, Quebec, Canada.
FAU - Provost, Chantale
AU  - Provost C
AD  - Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Swine and 
      Poultry Infectious Diseases Research Center (CRIPA), Saint-Hyacinthe, Quebec, 
      Canada.
FAU - Gagnon, Carl A
AU  - Gagnon CA
AD  - Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Swine and 
      Poultry Infectious Diseases Research Center (CRIPA), Saint-Hyacinthe, Quebec, 
      Canada.
FAU - Chorfi, Younes
AU  - Chorfi Y
AD  - Departement de Biomedecine Veterinaire, Saint-Hyacinthe, Quebec, Canada. 
      Electronic address: Younes.chorfi@umontreal.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141030
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (Mycotoxins)
RN  - 0 (Trichothecenes)
RN  - JT37HYP23V (deoxynivalenol)
SB  - IM
MH  - Animal Feed
MH  - Animals
MH  - Antibodies, Viral/*drug effects
MH  - Immunity, Mucosal/drug effects
MH  - Mycotoxins/*pharmacology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*drug 
      effects/immunology/physiology
MH  - Swine
MH  - Swine Diseases/*immunology/virology
MH  - Trichothecenes/*pharmacology
MH  - Virus Replication
OTO - NOTNLM
OT  - DON mycotoxin
OT  - PRRSV
OT  - Pig
OT  - Predisposition to infection
EDAT- 2014/12/04 06:00
MHDA- 2016/05/03 06:00
CRDT- 2014/12/04 06:00
PHST- 2014/09/02 00:00 [received]
PHST- 2014/10/17 00:00 [revised]
PHST- 2014/10/21 00:00 [accepted]
PHST- 2014/12/04 06:00 [entrez]
PHST- 2014/12/04 06:00 [pubmed]
PHST- 2016/05/03 06:00 [medline]
AID - S0378-1135(14)00497-0 [pii]
AID - 10.1016/j.vetmic.2014.10.019 [doi]
PST - ppublish
SO  - Vet Microbiol. 2014 Dec 5;174(3-4):419-426. doi: 10.1016/j.vetmic.2014.10.019. 
      Epub 2014 Oct 30.