PMID- 25467798
OWN - NLM
STAT- MEDLINE
DCOM- 20150304
LR  - 20150102
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 64
IP  - 1
DP  - 2015 Mar
TI  - Activation of calcium-sensing receptor increases TRPC3/6 expression in T 
      lymphocyte in sepsis.
PG  - 18-25
LID - S0161-5890(14)00283-1 [pii]
LID - 10.1016/j.molimm.2014.10.018 [doi]
AB  - Sepsis is a systemic inflammatory response syndrome induced by infection. T 
      Lymphocytes play an important role in this disease. Transient receptor potential 
      (TRP) channels and calcium-sensing receptors (CaSR) are expressed in lymphocytes 
      to promote intracellular Ca(2+) release. However, data about the link between 
      CaSR and TRP channels in septic T lymphocytes are few. In this study, by Ca(2+) 
      imaging and Western blotting, we found that in septic rat peripheral blood T 
      lymphocytes expressions of TRPC3 and TRPC6 proteins are higher. The SR/ER Ca(2+) 
      ATPase inhibitor thapsigargin (TG) and CaSR agonist NPS R-568 also increased 
      expressions of TRPC3 and TRPC6 proteins, which were reversed by PLC-IP3 channel 
      blocker U73122 and TRPC channels inhibitor SKF96365. By Ca(2+) imaging, we found 
      that the depletion of ER Ca(2+) stores by TG elicited a transient rise in 
      cytoplasmic Ca(2+), followed by sustained increase depending on extracellular 
      Ca(2+). But, SKF96365, not Verapamil (L-type channels inhibitor) and NiCl2 
      (Na(+)/Ca(2+) exchanger inhibitor), inhibited the relatively high [Ca(2+)]i. NPS 
      R-568 also resulted in the same effect, and the duration of [Ca(2+)]i increase 
      was eliminated completely by U73122 and was reduced in the absence of [Ca(2+)]o. 
      NPS R-568 and TG increased the apoptotic ratio of septic T lymphocytes, which can 
      be suppressed by SKF96365 and U73122. These results suggested that CaSR 
      activation promoted the expression of TRPC3 and TRPC6 and enhanced T lymphocytes 
      apoptosis through PLC-IP3 signaling pathway in sepsis.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Wu, Qiu-yue
AU  - Wu QY
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Harbin 
      Medical University, Harbin 150086, China.
FAU - Sun, Ming-rui
AU  - Sun MR
AD  - Department of Pharmacology, Qiqihaer Medical College, Qiqihaer 160001, China.
FAU - Wu, Chun-li
AU  - Wu CL
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Harbin 
      Medical University, Harbin 150086, China.
FAU - Li, Yang
AU  - Li Y
AD  - Department of Rheumatology, the Second Affiliated Hospital of Harbin Medical 
      University, Harbin 150086, China.
FAU - Du, Jing-jing
AU  - Du JJ
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Harbin 
      Medical University, Harbin 150086, China.
FAU - Zeng, Jing-ya
AU  - Zeng JY
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Harbin 
      Medical University, Harbin 150086, China.
FAU - Bi, Hai-liang
AU  - Bi HL
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Harbin 
      Medical University, Harbin 150086, China.
FAU - Sun, Yi-hua
AU  - Sun YH
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Harbin 
      Medical University, Harbin 150086, China. Electronic address: syh200415@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141112
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Receptors, Calcium-Sensing)
RN  - 0 (TRPC Cation Channels)
RN  - 0 (TRPC3 cation channel)
RN  - 0 (Trpc6 protein, rat)
RN  - 67526-95-8 (Thapsigargin)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - EC 3.1.4.- (Type C Phospholipases)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Calcium Signaling/drug effects
MH  - Extracellular Space/drug effects/metabolism
MH  - Flow Cytometry
MH  - Inositol 1,4,5-Trisphosphate/metabolism
MH  - Intracellular Space/drug effects/metabolism
MH  - Ion Channel Gating/drug effects
MH  - Rats, Wistar
MH  - Receptors, Calcium-Sensing/agonists/*metabolism
MH  - Sepsis/blood/*immunology/*pathology
MH  - T-Lymphocytes/cytology/drug effects/*metabolism
MH  - TRPC Cation Channels/*metabolism
MH  - Thapsigargin/pharmacology
MH  - Type C Phospholipases/metabolism
OTO - NOTNLM
OT  - Calcium-sensing receptor
OT  - Cytokine
OT  - Lymphocytes
OT  - Sepsis
OT  - Transient receptor potential channel
EDAT- 2014/12/04 06:00
MHDA- 2015/03/05 06:00
CRDT- 2014/12/04 06:00
PHST- 2014/09/19 00:00 [received]
PHST- 2014/10/23 00:00 [accepted]
PHST- 2014/12/04 06:00 [entrez]
PHST- 2014/12/04 06:00 [pubmed]
PHST- 2015/03/05 06:00 [medline]
AID - S0161-5890(14)00283-1 [pii]
AID - 10.1016/j.molimm.2014.10.018 [doi]
PST - ppublish
SO  - Mol Immunol. 2015 Mar;64(1):18-25. doi: 10.1016/j.molimm.2014.10.018. Epub 2014 
      Nov 12.