PMID- 2546911
OWN - NLM
STAT- MEDLINE
DCOM- 19890829
LR  - 20190723
IS  - 0021-8820 (Print)
IS  - 0021-8820 (Linking)
VI  - 42
IP  - 7
DP  - 1989 Jul
TI  - Inhibitory effect of antibiotic cerulenin on the respiratory burst in phagocytes. 
      II. Inhibition by cerulenin of intracellular calcium mobilization in human 
      neutrophils.
PG  - 1178-83
AB  - Generation of superoxide anion (O2-) and mobilization of intracellular Ca2+ in 
      human neutrophils upon exposure to stimuli such as 
      N-formylmethionylleucylphenylalanine (fMLP) were inhibited by the antibiotic 
      cerulenin, which inhibits fatty acid and cholesterol biosynthesis. The inhibition 
      of O2- generation and Ca2+-mobilization required certain periods of incubation 
      with cerulenin and both abilities of the cells were gradually lost following a 
      similar time-course. In contrast, significant Ca2+-influx from the medium was 
      observed in cerulenin-treated cells as well as untreated cells. The results 
      suggest that an event which coincides with the Ca2+-mobilization and not Ca2+ per 
      se is important for the induction of O2- generation in the fMLP-stimulated cells 
      and that this step is blocked in cerulenin-treated cells. Phorbol myristate 
      acetate or synthetic 1-oleoyl-2-acetylglycerol were able to bypass the block and 
      induced O2- generation in cerulenin-treated cells.
FAU - Nakata, M
AU  - Nakata M
AD  - Institute of Medical Science, University of Tokyo, Japan.
FAU - Tomita, T
AU  - Tomita T
FAU - Iizuka, T
AU  - Iizuka T
FAU - Kanegasaki, S
AU  - Kanegasaki S
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Antibiot (Tokyo)
JT  - The Journal of antibiotics
JID - 0151115
RN  - 0 (Antifungal Agents)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Diglycerides)
RN  - 0 (staphylococcal delta toxin)
RN  - 11062-77-4 (Superoxides)
RN  - 17397-89-6 (Cerulenin)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 86390-77-4 (1-oleoyl-2-acetylglycerol)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - S88TT14065 (Oxygen)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Antifungal Agents/*pharmacology
MH  - Bacterial Toxins/pharmacology
MH  - Calcimycin/pharmacology
MH  - Calcium/*metabolism
MH  - Cerulenin/*pharmacology
MH  - Diglycerides/pharmacology
MH  - Humans
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*drug effects/metabolism
MH  - Oxygen/metabolism
MH  - Phagocytes/*drug effects
MH  - Phagocytosis/*drug effects
MH  - Sodium Fluoride/pharmacology
MH  - Superoxides/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1989/07/01 00:00
MHDA- 1989/07/01 00:01
CRDT- 1989/07/01 00:00
PHST- 1989/07/01 00:00 [pubmed]
PHST- 1989/07/01 00:01 [medline]
PHST- 1989/07/01 00:00 [entrez]
AID - 10.7164/antibiotics.42.1178 [doi]
PST - ppublish
SO  - J Antibiot (Tokyo). 1989 Jul;42(7):1178-83. doi: 10.7164/antibiotics.42.1178.