PMID- 25469646
OWN - NLM
STAT- MEDLINE
DCOM- 20150924
LR  - 20220330
IS  - 1365-2222 (Electronic)
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 45
IP  - 2
DP  - 2015 Feb
TI  - Elevated presence of myeloid dendritic cells in nasal polyps of patients with 
      chronic rhinosinusitis.
PG  - 384-93
LID - 10.1111/cea.12471 [doi]
AB  - BACKGROUND: Although chronic rhinosinusitis with nasal polyps (CRSwNP) is 
      characterized by Th2 inflammation, the mechanism underlying the onset and 
      amplification of this inflammation has not been fully elucidated. Dendritic cells 
      (DCs) are major antigen-presenting cells, central inducers of adaptive immunity 
      and critical regulators of many inflammatory diseases. However, the presence of 
      DCs in CRS, especially in nasal polyps (NPs), has not been extensively studied. 
      OBJECTIVE: The objective of this study was to characterize DC subsets in CRS. 
      METHODS: We used real-time PCR to assess the expression of mRNA for markers of 
      myeloid DCs (mDCs; CD1c), plasmacytoid DCs (pDCs; CD303) and Langerhans cells 
      (LCs; CD1a, CD207) in uncinate tissue (UT) from controls and patients with CRS as 
      well as in NP. We assayed the presence of DCs by immunohistochemistry and flow 
      cytometry. RESULTS: Compared to UT from control subjects (n = 15) and patients 
      with CRS without NP (CRSsNP) (n = 16) and CRSwNP (n = 17), mRNAs for CD1a and 
      CD1c were significantly elevated in NPs (n = 29). In contrast, CD207 mRNA was not 
      elevated in NPs. Immunohistochemistry showed that CD1c(+) cells but not CD303(+) 
      cells were significantly elevated in NPs compared to control subjects or patients 
      with CRSsNP. Flow cytometric analysis showed that CD1a(+) cells in NPs might be a 
      subset of mDC1s and that CD45(+) CD19(-) CD1c(+) CD11c(+) CD141(-) CD303(-) 
      HLA-DR(+) mDC1s and CD45(+) CD19(-) CD11c(+) CD1c(-) CD141(high) HLA-DR(+) mDC2s 
      were significantly elevated in NPs compared to UT from controls and CRSsNP, but 
      CD45(+) CD11c(-) CD303(+) HLA-DR(+) pDCs were only elevated in NPs compared to 
      control UT. CONCLUSION AND CLINICAL RELEVANCE: Myeloid DCs are elevated in 
      CRSwNP, especially in NPs. Myeloid DCs thus may indirectly contribute to the 
      inflammation observed in CRSwNP.
CI  - (c) 2014 John Wiley & Sons Ltd.
FAU - Poposki, J A
AU  - Poposki JA
AD  - Division of Allergy-Immunology, Department of Medicine, Northwestern University 
      Feinberg School of Medicine, Chicago, IL, USA.
FAU - Peterson, S
AU  - Peterson S
FAU - Welch, K
AU  - Welch K
FAU - Schleimer, R P
AU  - Schleimer RP
FAU - Hulse, K E
AU  - Hulse KE
FAU - Peters, A T
AU  - Peters AT
FAU - Norton, J
AU  - Norton J
FAU - Suh, L A
AU  - Suh LA
FAU - Carter, R
AU  - Carter R
FAU - Harris, K E
AU  - Harris KE
FAU - Grammer, L C
AU  - Grammer LC
FAU - Tan, B K
AU  - Tan BK
FAU - Chandra, R K
AU  - Chandra RK
FAU - Conley, D B
AU  - Conley DB
FAU - Kern, R C
AU  - Kern RC
FAU - Kato, A
AU  - Kato A
LA  - eng
GR  - R01 HL078860/HL/NHLBI NIH HHS/United States
GR  - U19 AI106683/AI/NIAID NIH HHS/United States
GR  - R21 HL113913/HL/NHLBI NIH HHS/United States
GR  - K12 HD055884/HD/NICHD NIH HHS/United States
GR  - R37 HL068546/HL/NHLBI NIH HHS/United States
GR  - R01 AI104733/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Antigens, Surface)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, Surface/genetics/metabolism
MH  - Biomarkers
MH  - Chronic Disease
MH  - Dendritic Cells/*immunology/metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Male
MH  - Middle Aged
MH  - Myeloid Cells/*immunology/metabolism
MH  - Nasal Polyps/complications/*immunology/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Rhinitis/complications/*immunology/metabolism
MH  - Sinusitis/complications/*immunology/metabolism
MH  - Th2 Cells/immunology/metabolism
MH  - Young Adult
PMC - PMC4467201
MID - NIHMS698131
OTO - NOTNLM
OT  - chronic rhinosinusitis
OT  - myeloid dendritic cells
OT  - nasal polyps
OT  - plasmacytoid dendritic cells
COIS- Conflict of interest The authors declare no conflict of interest as to the 
      interpretation and presentation of this manuscript.
EDAT- 2014/12/04 06:00
MHDA- 2015/09/25 06:00
PMCR- 2016/02/01
CRDT- 2014/12/04 06:00
PHST- 2014/08/09 00:00 [received]
PHST- 2014/11/23 00:00 [revised]
PHST- 2014/11/28 00:00 [accepted]
PHST- 2014/12/04 06:00 [entrez]
PHST- 2014/12/04 06:00 [pubmed]
PHST- 2015/09/25 06:00 [medline]
PHST- 2016/02/01 00:00 [pmc-release]
AID - 10.1111/cea.12471 [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2015 Feb;45(2):384-93. doi: 10.1111/cea.12471.