PMID- 25470679
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141209
LR  - 20190518
IS  - 0261-1929 (Print)
IS  - 0261-1929 (Linking)
VI  - 27
IP  - 3
DP  - 1999 May-Jun
TI  - Apoptosis Versus Cytotoxicity in HeLa Cells Exposed to Paracetamol.
PG  - 403-12
AB  - Apoptosis is a programmed form of cell death which occurs in response to specific 
      stimuli. It is distinguished from necrotic or accidental cell death by unique 
      events, including the degradation of chromatin and a loss of cellular volume. In 
      contrast to necrotic cell death, cell membrane integrity and mitochondrial 
      function are thought to be maintained until the apoptotic process is well 
      advanced. One of the novel assays for detecting apoptosis is flow cytometry. In 
      our experiments, we used a flow cytometric assay to detect DNA changes in a human 
      cell line (HeLa) exposed to paracetamol, by measuring propidium iodide binding. 
      We were able to detect the apoptotic process in cells exposed to paracetamol. 
      Apoptosis did not correlate with cytotoxicity, and was only found in samples 
      exposed to 4-5mg/ml paracetamol for 8 hours in minimum essential medium and 
      incubated in fresh medium without paracetamol for 14-19 hours. The greatest 
      effect was noted 18 hours after paracetamol exposure. These results were 
      confirmed by studying cell morphology and chromatin condensation by fluorescent 
      microscopy with the fluorochromes acridine orange and ethidium bromide. Our 
      results support the hypothesis that, in cultured cells, apoptosis is induced by a 
      relatively narrow range of chemical concentrations which are known to inhibit the 
      cell cycle, and that apoptosis and inhibition of cell proliferation coincide to 
      some degree.
CI  - 1999 FRAME.
FAU - Ruppova, K
AU  - Ruppova K
AD  - Institute of Preventive and Clinical Medicine, Limbova 14, 833 01 Bratislava, 
      Slovakia.
FAU - Urbancikova, M
AU  - Urbancikova M
AD  - Institute of Preventive and Clinical Medicine, Limbova 14, 833 01 Bratislava, 
      Slovakia.
FAU - Wsolova, L
AU  - Wsolova L
AD  - Institute of Preventive and Clinical Medicine, Limbova 14, 833 01 Bratislava, 
      Slovakia.
FAU - Slamenova, D
AU  - Slamenova D
AD  - Cancer Research Institute, Slovak Academy of Sciences, Vlarska 7, 833 01 
      Bratislava, Slovakia.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Altern Lab Anim
JT  - Alternatives to laboratory animals : ATLA
JID - 8110074
EDAT- 1999/05/01 00:00
MHDA- 1999/05/01 00:01
CRDT- 2014/12/04 06:00
PHST- 2014/12/04 06:00 [entrez]
PHST- 1999/05/01 00:00 [pubmed]
PHST- 1999/05/01 00:01 [medline]
AID - 10.1177/026119299902700316 [doi]
PST - ppublish
SO  - Altern Lab Anim. 1999 May-Jun;27(3):403-12. doi: 10.1177/026119299902700316.