PMID- 25471622
OWN - NLM
STAT- MEDLINE
DCOM- 20150226
LR  - 20220316
IS  - 1573-2959 (Electronic)
IS  - 0167-6369 (Linking)
VI  - 187
IP  - 1
DP  - 2015 Jan
TI  - Differential expression of HO-1 and CYP1A2 during up-regulation of ERK in 
      stressed fish hepatocytes.
PG  - 4147
LID - 10.1007/s10661-014-4147-1 [doi]
AB  - Induction of heme oxygenase-1 (HO-1) and extracellular signal-regulated kinase 
      (ERK) is a crucial step in the cellular response to oxidative stress. Altered 
      expression of such proteins in response to stress conditions is a key factor for 
      the maintenance of cellular integrity and survival. Hypoxia-inducible factor-1 
      (HIF-1) and cytochrome P450 1A (CYP1A) are critical indicators of environmental 
      exposure to hypoxia. The current study was aimed to analyse the expression 
      pattern of HO-1, p-ERK, HIF-1alpha and CYP1A2 in hepatocytes of Mugil cephalus 
      inhabiting Kovalam (unpolluted site) and Ennore (polluted site) estuaries. The 
      impact of stress on hepatocytes was assessed by measuring the level of lipid 
      hydroperoxides (LHP) and reduced glutathione (GSH) in hepatocytes of M. cephalus 
      inhabiting these estuaries. The expression of HO-1, p-ERK, HIF-1alpha and CYP1A2 was 
      analysed by ELISA and immunoblot analysis, respectively. There was significant 
      decrease of GSH (19%) and CYP1A2 (34%) along with significant increase of LHP 
      (31%), HO-1 (96%), p-ERK (p < 0.01) and HIF-1alpha (41%) in hepatocytes of M. 
      cephalus inhabiting Ennore estuary than Kovalam estuary. The present study shows 
      that increased synthesis of HO-1 mainly regulated by p-ERK and HIF-1alpha may 
      significantly contribute to the environmental adaptation processes of grey 
      mullet, by protecting the cells from oxidative stress and stress-induced 
      degenerative changes.
FAU - Padmini, Ekambaram
AU  - Padmini E
AD  - P.G. Department of Biochemistry, Bharathi Women's College, Affiliated to 
      University of Madras, Chennai, 600108, Tamil Nadu, India, 
      dstpadmini@rediffmail.com.
FAU - Tharani, Jayachandran
AU  - Tharani J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141204
PL  - Netherlands
TA  - Environ Monit Assess
JT  - Environmental monitoring and assessment
JID - 8508350
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A2)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Cytochrome P-450 CYP1A2/genetics/*metabolism
MH  - Cytochrome P-450 Enzyme System/metabolism
MH  - Environmental Monitoring
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Glutathione/metabolism
MH  - Heme Oxygenase-1/genetics/*metabolism
MH  - Hepatocytes/*metabolism
MH  - Oxidation-Reduction
MH  - Oxidative Stress/physiology
MH  - Smegmamorpha/metabolism
MH  - Up-Regulation
EDAT- 2014/12/05 06:00
MHDA- 2015/02/27 06:00
CRDT- 2014/12/05 06:00
PHST- 2014/06/06 00:00 [received]
PHST- 2014/11/03 00:00 [accepted]
PHST- 2014/12/05 06:00 [entrez]
PHST- 2014/12/05 06:00 [pubmed]
PHST- 2015/02/27 06:00 [medline]
AID - 10.1007/s10661-014-4147-1 [doi]
PST - ppublish
SO  - Environ Monit Assess. 2015 Jan;187(1):4147. doi: 10.1007/s10661-014-4147-1. Epub 
      2014 Dec 4.