PMID- 25471636
OWN - NLM
STAT- MEDLINE
DCOM- 20151109
LR  - 20181113
IS  - 1471-2156 (Electronic)
IS  - 1471-2156 (Linking)
VI  - 15 Suppl 2
IP  - Suppl 2
DP  - 2014
TI  - The Bactrocera dorsalis species complex: comparative cytogenetic analysis in 
      support of Sterile Insect Technique applications.
PG  - S16
LID - 10.1186/1471-2156-15-S2-S16 [doi]
AB  - BACKGROUND: The Bactrocera dorsalis species complex currently harbors 
      approximately 90 different members. The species complex has undergone many 
      revisions in the past decades, and there is still an ongoing debate about the 
      species limits. The availability of a variety of tools and approaches, such as 
      molecular-genomic and cytogenetic analyses, are expected to shed light on the 
      rather complicated issues of species complexes and incipient speciation. The 
      clarification of genetic relationships among the different members of this 
      complex is a prerequisite for the rational application of sterile insect 
      technique (SIT) approaches for population control. RESULTS: Colonies established 
      in the Insect Pest Control Laboratory (IPCL) (Seibersdorf, Vienna), representing 
      five of the main economic important members of the Bactrocera dorsalis complex 
      were cytologically characterized. The taxa under study were B. dorsalis s.s., B. 
      philippinensis, B. papayae, B. invadens and B. carambolae. Mitotic and polytene 
      chromosome analyses did not reveal any chromosomal characteristics that could be 
      used to distinguish between the investigated members of the B. dorsalis complex. 
      Therefore, their polytene chromosomes can be regarded as homosequential with the 
      reference maps of B. dorsalis s.s.. In situ hybridization of six genes further 
      supported the proposed homosequentiallity of the chromosomes of these specific 
      members of the complex. CONCLUSIONS: The present analysis supports that the 
      polytene chromosomes of the five taxa under study are homosequential. Therefore, 
      the use of the available polytene chromosome maps for B. dorsalis s.s. as 
      reference maps for all these five biological entities is proposed. Present data 
      provide important insight in the genetic relationships among the different 
      members of the B. dorsalis complex, and, along with other studies in the field, 
      can facilitate SIT applications targeting this complex. Moreover, the 
      availability of 'universal' reference polytene chromosome maps for members of the 
      complex, along with the documented application of in situ hybridization, can 
      facilitate ongoing and future genome projects in this complex.
FAU - Augustinos, Antonios A
AU  - Augustinos AA
FAU - Drosopoulou, Elena
AU  - Drosopoulou E
FAU - Gariou-Papalexiou, Aggeliki
AU  - Gariou-Papalexiou A
FAU - Bourtzis, Kostas
AU  - Bourtzis K
FAU - Mavragani-Tsipidou, Penelope
AU  - Mavragani-Tsipidou P
FAU - Zacharopoulou, Antigone
AU  - Zacharopoulou A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141201
PL  - England
TA  - BMC Genet
JT  - BMC genetics
JID - 100966978
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - Chromosomes, Insect
MH  - Cytogenetic Analysis
MH  - Female
MH  - In Situ Hybridization
MH  - Insect Control/methods
MH  - Karyotype
MH  - Male
MH  - Polytene Chromosomes
MH  - Tephritidae/*classification/*genetics
PMC - PMC4255788
EDAT- 2014/12/05 06:00
MHDA- 2015/11/10 06:00
PMCR- 2014/12/01
CRDT- 2014/12/05 06:00
PHST- 2014/12/05 06:00 [entrez]
PHST- 2014/12/05 06:00 [pubmed]
PHST- 2015/11/10 06:00 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - 1471-2156-15-S2-S16 [pii]
AID - 10.1186/1471-2156-15-S2-S16 [doi]
PST - ppublish
SO  - BMC Genet. 2014;15 Suppl 2(Suppl 2):S16. doi: 10.1186/1471-2156-15-S2-S16. Epub 
      2014 Dec 1.