PMID- 2547206
OWN - NLM
STAT- MEDLINE
DCOM- 19890901
LR  - 20190818
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 10
IP  - 2
DP  - 1989 Mar-Apr
TI  - Functional corticotropin-releasing factor (CRF) receptors in mouse spleen: 
      evidence from adenylate cyclase studies.
PG  - 395-401
AB  - Radioligand binding studies have previously identified a high affinity, 
      magnesium-dependent, guanine nucleotide-sensitive binding site for 
      corticotropin-releasing factor (CRF) in mouse spleen. In order to determine the 
      functional nature of these CRF binding sites, we examined the effects of CRF on 
      adenylate cyclase activity in mouse spleen homogenates. The stimulation of 
      adenylate cyclase activity was dependent on time, tissue protein concentration, 
      and guanine nucleotides. CRF-stimulated adenylate cyclase activity was evident in 
      the presence of guanosine-5'-triphosphate (GTP) and its precursor 
      guanosine-5'-diphosphate (GDP) but was not detected in the presence of the 
      hydrolysis-resistant GTP analogs, guanyl-5'-imidodiphosphate [Gpp(NH)p] and 
      guanosine-5'-gamma-thiotriphosphate (GTP-gamma-S). The rank order of potency for 
      CRF analogs and fragments in stimulating adenylate cyclase activity was 
      comparable to their affinities for CRF binding sites in mouse spleen homogenates. 
      The putative receptor antagonist, alpha helical ovine CRF(9-41), did not 
      stimulate adenylate cyclase activity but did attenuate the stimulation by various 
      concentrations of rat/human CRF. In summary, these data demonstrate the 
      functional nature of CRF receptors in mouse spleen as evidenced by CRF 
      stimulation of cAMP production and suggest that this peptide may play a 
      physiological role in regulating immune function.
FAU - Webster, E L
AU  - Webster EL
AD  - Neuroscience Branch, National Institute on Drug Abuse, Baltimore, MD 21224.
FAU - Battaglia, G
AU  - Battaglia G
FAU - De Souza, E B
AU  - De Souza EB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Receptors, Corticotropin-Releasing Hormone)
RN  - 0 (Receptors, Neurotransmitter)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Thionucleotides)
RN  - 34273-04-6 (Guanylyl Imidodiphosphate)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclases/*metabolism
MH  - Animals
MH  - Brain/enzymology
MH  - Corticotropin-Releasing Hormone/*metabolism/*pharmacology
MH  - Guanosine 5'-O-(3-Thiotriphosphate)
MH  - Guanosine Triphosphate/analogs & derivatives/pharmacology
MH  - Guanylyl Imidodiphosphate/pharmacology
MH  - Kinetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Receptors, Corticotropin-Releasing Hormone
MH  - Receptors, Neurotransmitter/*metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Spleen/enzymology/*metabolism
MH  - Thionucleotides/pharmacology
EDAT- 1989/03/01 00:00
MHDA- 1989/03/01 00:01
CRDT- 1989/03/01 00:00
PHST- 1989/03/01 00:00 [pubmed]
PHST- 1989/03/01 00:01 [medline]
PHST- 1989/03/01 00:00 [entrez]
AID - 0196-9781(89)90049-1 [pii]
AID - 10.1016/0196-9781(89)90049-1 [doi]
PST - ppublish
SO  - Peptides. 1989 Mar-Apr;10(2):395-401. doi: 10.1016/0196-9781(89)90049-1.