PMID- 25475309
OWN - NLM
STAT- MEDLINE
DCOM- 20150717
LR  - 20191210
IS  - 1095-9998 (Electronic)
IS  - 0740-0020 (Linking)
VI  - 46
DP  - 2015 Apr
TI  - FISHing for bacteria in food--a promising tool for the reliable detection of 
      pathogenic bacteria?
PG  - 395-407
LID - S0740-0020(14)00231-7 [pii]
LID - 10.1016/j.fm.2014.09.002 [doi]
AB  - Foodborne pathogens cause millions of infections every year and are responsible 
      for considerable economic losses worldwide. The current gold standard for the 
      detection of bacterial pathogens in food is still the conventional cultivation 
      following standardized and generally accepted protocols. However, these methods 
      are time-consuming and do not provide fast information about food contaminations 
      and thus are limited in their ability to protect consumers in time from potential 
      microbial hazards. Fluorescence in situ hybridization (FISH) represents a rapid 
      and highly specific technique for whole-cell detection. This review aims to 
      summarize the current data on FISH-testing for the detection of pathogenic 
      bacteria in different food matrices and to evaluate its suitability for the 
      implementation in routine testing. In this context, the use of FISH in different 
      matrices and their pretreatment will be presented, the sensitivity and 
      specificity of FISH tests will be considered and the need for automation shall be 
      discussed as well as the use of technological improvements to overcome current 
      hurdles for a broad application in monitoring food safety. In addition, the 
      overall economical feasibility will be assessed in a rough calculation of costs, 
      and strengths and weaknesses of FISH are considered in comparison with 
      traditional and well-established detection methods.
CI  - Copyright (c) 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Rohde, Alexander
AU  - Rohde A
AD  - Federal Institute for Risk Assessment, Department of Biological Safety, D-12277 
      Berlin, Germany. Electronic address: alexander.rohde@bfr.bund.de.
FAU - Hammerl, Jens Andre
AU  - Hammerl JA
AD  - Federal Institute for Risk Assessment, Department of Biological Safety, D-12277 
      Berlin, Germany.
FAU - Appel, Bernd
AU  - Appel B
AD  - Federal Institute for Risk Assessment, Department of Biological Safety, D-12277 
      Berlin, Germany.
FAU - Dieckmann, Ralf
AU  - Dieckmann R
AD  - Federal Institute for Risk Assessment, Department of Biological Safety, D-12277 
      Berlin, Germany.
FAU - Al Dahouk, Sascha
AU  - Al Dahouk S
AD  - Federal Institute for Risk Assessment, Department of Biological Safety, D-12277 
      Berlin, Germany.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20140916
PL  - England
TA  - Food Microbiol
JT  - Food microbiology
JID - 8601127
SB  - IM
MH  - Bacteria/genetics/*isolation & purification
MH  - *Food Microbiology
MH  - Foodborne Diseases/*microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - Food safety
OT  - Foodborne illnesses
OT  - Rapid detection methods
OT  - Ribosomal RNA
EDAT- 2014/12/06 06:00
MHDA- 2015/07/18 06:00
CRDT- 2014/12/06 06:00
PHST- 2014/04/29 00:00 [received]
PHST- 2014/08/15 00:00 [revised]
PHST- 2014/09/05 00:00 [accepted]
PHST- 2014/12/06 06:00 [entrez]
PHST- 2014/12/06 06:00 [pubmed]
PHST- 2015/07/18 06:00 [medline]
AID - S0740-0020(14)00231-7 [pii]
AID - 10.1016/j.fm.2014.09.002 [doi]
PST - ppublish
SO  - Food Microbiol. 2015 Apr;46:395-407. doi: 10.1016/j.fm.2014.09.002. Epub 2014 Sep 
      16.