PMID- 25475842
OWN - NLM
STAT- MEDLINE
DCOM- 20151120
LR  - 20150303
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 74
DP  - 2015 Mar
TI  - Response surface optimization and physicochemical properties of polysaccharides 
      from Nelumbo nucifera leaves.
PG  - 103-10
LID - S0141-8130(14)00778-8 [pii]
LID - 10.1016/j.ijbiomac.2014.11.020 [doi]
AB  - Dynamic high pressure microfluidization (DHPM)-assisted extraction (DHPMAE) of 
      lotus (Nelumbo nucifera) leaves polysaccharides (LLPs) was optimized by response 
      surface methodology. The optimal extraction conditions were: liquid/solid ratio 
      of 35:1 (v/m, mL/g), processing pressure of 180 MPa, processed two times, 
      extraction temperature of 76 degrees C, extraction time of 50 min. Under the optimal 
      extraction conditions, DHPMAE produced a higher polysaccharides yield (6.31%) 
      than leaching (2.95%). Scanning electron microscope (SEM) analysis revealed that 
      DHPM could reduce the particles size and make the surface more unconsolidated. 
      The LLPs prepared by both methods showed similar FT-IR spectrum, and were 
      consisted of the same monosaccharides, including rhamnose, fucose, arabinose, 
      xylose, mannose, glucose and galactose. The content of each monosaccharide in 
      extracts, however, was quite different. The average molecular weight of LLPs 
      prepared by DHPMAE is 550 kDa, smaller than 578 kDa obtained by leaching. The 
      LLPs prepared by DHPMAE exhibited stronger DPPH scavenging ability (IC50 value of 
      0.38 mg/mL), HO scavenging ability (IC50 value of 0.61 mg/mL) and reducing power. 
      Therefore, DHPMAE can be a promising alternative to traditional extraction 
      techniques for polysaccharides from plants, and lotus leaves might be a potential 
      resource of natural antioxidants.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Zhang, Lu
AU  - Zhang L
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, Jiangxi 330047, China.
FAU - Tu, Zong-cai
AU  - Tu ZC
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, Jiangxi 330047, China; Jiangxi Normal University, Nanchang, Jiangxi 
      330022, China. Electronic address: Tuzc_mail@aliyun.com.
FAU - Wang, Hui
AU  - Wang H
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, Jiangxi 330047, China. Electronic address: Wanghui00072@aliyun.com.
FAU - Kou, Yu
AU  - Kou Y
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, Jiangxi 330047, China.
FAU - Wen, Qing-hui
AU  - Wen QH
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, Jiangxi 330047, China.
FAU - Fu, Zhi-feng
AU  - Fu ZF
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, Jiangxi 330047, China.
FAU - Chang, Hai-xia
AU  - Chang HX
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, Jiangxi 330047, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141202
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antioxidants)
RN  - 0 (Plant Extracts)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Antioxidants/chemistry/pharmacology
MH  - Molecular Weight
MH  - Nelumbo/*chemistry
MH  - Oxidation-Reduction/drug effects
MH  - Plant Extracts/*chemistry/pharmacology
MH  - Plant Leaves/*chemistry
MH  - Polysaccharides/*chemistry/pharmacology
MH  - Spectroscopy, Fourier Transform Infrared
OTO - NOTNLM
OT  - Nelumbo nucifera leaves
OT  - Polysaccharides
OT  - Response surface methodology
EDAT- 2014/12/06 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/12/06 06:00
PHST- 2014/10/08 00:00 [received]
PHST- 2014/11/11 00:00 [revised]
PHST- 2014/11/16 00:00 [accepted]
PHST- 2014/12/06 06:00 [entrez]
PHST- 2014/12/06 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0141-8130(14)00778-8 [pii]
AID - 10.1016/j.ijbiomac.2014.11.020 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2015 Mar;74:103-10. doi: 10.1016/j.ijbiomac.2014.11.020. 
      Epub 2014 Dec 2.