PMID- 25475961 OWN - NLM STAT- MEDLINE DCOM- 20151023 LR - 20150130 IS - 1879-0089 (Electronic) IS - 0145-305X (Linking) VI - 49 IP - 2 DP - 2015 Apr TI - Molecular and functional characterization of an IL-1beta receptor antagonist in grass carp (Ctenopharyngodon idella). PG - 207-16 LID - S0145-305X(14)00298-5 [pii] LID - 10.1016/j.dci.2014.11.016 [doi] AB - In the present study, we discovered a novel IL-1 family member (nIL-1F) from grass carp that possessed the ability to bind with grass carp IL-1beta receptor type 1 (gcIL-1R1) and attenuate grass carp IL-1beta activity in head kidney leukocytes (HKLs), suggesting that it may function as an IL-1beta receptor antagonist. Grass carp nIL-1F transcript was constitutively expressed with the highest levels in some lymphoid organs, including head kidney, spleen and intestine, implying its potential in grass carp immunity. In agreement with this notion, in vitro and in vivo studies showed that nIL-1F mRNA was inductively expressed in grass carp with a rapid kinetics, indicating that it may be an early response gene during immune challenges. In addition, recombinant grass carp IL-1beta (rgcIL-1beta) induced nIL-1F mRNA expression via NF-kappaB and MAPK (JNK, p38 and p42/44) signaling pathways in HKLs. Particularly, the orthologs of nIL-1F found in other fish species, including zebrafish, pufferfish and rainbow trout are not homologous to mammalian IL-1beta receptor antagonist (IL-1Ra), indicating that fish nIL-1F and mammalian IL-1Ra may not share a common evolutionary ancestor. Taken together, our data suggest the existence of a naturally occurring fish nIL-1F, which may function like mammalian IL-1Ra, being beneficial to understand the auto-regulatory mechanism of IL-1beta activity in fish immunity. CI - Copyright (c) 2014 Elsevier Ltd. All rights reserved. FAU - Yao, Fuli AU - Yao F AD - School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. FAU - Yang, Xiao AU - Yang X AD - School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. FAU - Wang, Xinyan AU - Wang X AD - School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. FAU - Wei, He AU - Wei H AD - School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. FAU - Zhang, Anying AU - Zhang A AD - School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. FAU - Zhou, Hong AU - Zhou H AD - School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. Electronic address: hongzhou209@gmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141202 PL - United States TA - Dev Comp Immunol JT - Developmental and comparative immunology JID - 7708205 RN - 0 (Interleukin-1) RN - 0 (NF-kappa B) RN - 0 (Protein Isoforms) RN - 0 (RNA, Messenger) RN - 0 (Receptors, Interleukin-1) RN - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases) RN - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1) RN - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3) RN - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Carps MH - Cloning, Molecular MH - Gene Expression Profiling MH - Head Kidney/cytology/immunology MH - Interleukin-1/*pharmacology MH - JNK Mitogen-Activated Protein Kinases/metabolism MH - Leukocytes/cytology/*immunology MH - Mitogen-Activated Protein Kinase 1/metabolism MH - Mitogen-Activated Protein Kinase 3/metabolism MH - Molecular Sequence Data MH - NF-kappa B/metabolism MH - Phylogeny MH - Protein Isoforms/genetics MH - RNA, Messenger/biosynthesis MH - Receptors, Interleukin-1/*antagonists & inhibitors/*genetics MH - Sequence Alignment MH - Sequence Analysis, DNA MH - p38 Mitogen-Activated Protein Kinases/metabolism OTO - NOTNLM OT - Functional identification OT - Grass carp OT - Head kidney leukocytes OT - IL-1beta receptor antagonist OT - Receptor binding assay EDAT- 2014/12/06 06:00 MHDA- 2015/10/24 06:00 CRDT- 2014/12/06 06:00 PHST- 2014/07/25 00:00 [received] PHST- 2014/11/24 00:00 [revised] PHST- 2014/11/25 00:00 [accepted] PHST- 2014/12/06 06:00 [entrez] PHST- 2014/12/06 06:00 [pubmed] PHST- 2015/10/24 06:00 [medline] AID - S0145-305X(14)00298-5 [pii] AID - 10.1016/j.dci.2014.11.016 [doi] PST - ppublish SO - Dev Comp Immunol. 2015 Apr;49(2):207-16. doi: 10.1016/j.dci.2014.11.016. Epub 2014 Dec 2.