PMID- 25476271
OWN - NLM
STAT- MEDLINE
DCOM- 20150728
LR  - 20141205
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 132
DP  - 2015 Jan
TI  - Comparison of capillary electrophoresis-mass spectrometry and hydrophilic 
      interaction chromatography-mass spectrometry for anionic metabolic profiling of 
      urine.
PG  - 1-7
LID - S0039-9140(14)00727-9 [pii]
LID - 10.1016/j.talanta.2014.08.047 [doi]
AB  - In order to assess the utility of a recently developed capillary 
      electrophoresis-mass spectrometry (CE-MS) method for the study of anionic 
      metabolites in urine, a comparison was made with hydrophilic interaction 
      chromatography-MS (HILIC-MS) using negative electrospray ionization. After 
      optimization of the HILIC conditions, a gradient employing 10mM ammonium acetate 
      (pH 6.8) in acetonitrile-water (5 min 90% acetonitrile followed by 90%-50% 
      acetonitrile in 10 min) was selected, providing baseline separation of five 
      representative anionic test metabolites. Relative standard deviations (RSDs) for 
      HILIC retention times and peak areas were below 0.2% and 7.7%, respectively, and 
      detection limits were in the range 0.04-2.21 muM. Metabolites in rat urine could 
      also be analysed in a reproducible way with retention time and peak area RSDs 
      below 0.6% and 13.6%, respectively. The CE-MS and HILIC-MS methods were compared 
      in terms of reproducibility, sensitivity, selectivity and coverage of the anionic 
      urinary metabolome. In general, peak area RSDs were similar whereas HILIC-MS 
      yielded better retention-time repeatability and up to 80 times lower detection 
      limits (expressed in injected concentration) for test metabolites as compared to 
      CE-MS. Rat urine analysis by HILIC-MS provided detection of 1360 molecular 
      features compared to 347 molecular features revealed with CE-MS. Of these, a 
      number of 144 molecular features were found with both HILIC-MS and CE-MS, which 
      showed on average 10 times higher peak areas in HILIC-MS. The HILIC retention and 
      CE migration times of the common features were clearly not correlated. The HILIC 
      and CE behavior of the test metabolites and 16 putatively identified common 
      features were evaluated involving their physicochemical properties, indicating a 
      markedly different separation selectivity, and thus significant degree of 
      orthogonality of HILIC and CE.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Kok, Miranda G M
AU  - Kok MG
AD  - Biomolecular Analysis, Department of Pharmaceutical Sciences, Utrecht University, 
      P.O. Box 80082, 3508 TB Utrecht, The Netherlands; Research group Analysis 
      Techniques in Life Sciences, Avans Hogeschool, P.O. Box 90116, 4800 RA Breda, The 
      Netherlands. Electronic address: M.G.M.Kok@uu.nl.
FAU - Somsen, Govert W
AU  - Somsen GW
AD  - Biomolecular Analysis, Department of Pharmaceutical Sciences, Utrecht University, 
      P.O. Box 80082, 3508 TB Utrecht, The Netherlands; AIMSS Division of BioAnalytical 
      Chemistry, Department of Chemistry and Pharmaceutical Sciences, VU University, De 
      Boelelaan 1083, 1081 HV Amsterdam, The Netherlands.
FAU - de Jong, Gerhardus J
AU  - de Jong GJ
AD  - Biomolecular Analysis, Department of Pharmaceutical Sciences, Utrecht University, 
      P.O. Box 80082, 3508 TB Utrecht, The Netherlands; Research group Analysis 
      Techniques in Life Sciences, Avans Hogeschool, P.O. Box 90116, 4800 RA Breda, The 
      Netherlands.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20140827
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Acetates)
RN  - 0 (Acetonitriles)
RN  - 0 (Anions)
RN  - 059QF0KO0R (Water)
RN  - RRE756S6Q2 (ammonium acetate)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetates
MH  - Acetonitriles
MH  - Animals
MH  - Anions/*urine
MH  - Electrophoresis, Capillary/*instrumentation/methods/standards
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Limit of Detection
MH  - Mass Spectrometry/*instrumentation/methods/standards
MH  - Metabolome/physiology
MH  - Rats
MH  - Reference Standards
MH  - Spectrometry, Mass, Electrospray Ionization/instrumentation/methods
MH  - Urinalysis/*instrumentation/methods/standards
MH  - Water
OTO - NOTNLM
OT  - Anionic metabolic profiling
OT  - Capillary electrophoresis
OT  - Hydrophilic interaction chromatography
OT  - Mass spectrometry
OT  - Urine
EDAT- 2014/12/06 06:00
MHDA- 2015/07/29 06:00
CRDT- 2014/12/06 06:00
PHST- 2014/03/20 00:00 [received]
PHST- 2014/08/13 00:00 [revised]
PHST- 2014/08/18 00:00 [accepted]
PHST- 2014/12/06 06:00 [entrez]
PHST- 2014/12/06 06:00 [pubmed]
PHST- 2015/07/29 06:00 [medline]
AID - S0039-9140(14)00727-9 [pii]
AID - 10.1016/j.talanta.2014.08.047 [doi]
PST - ppublish
SO  - Talanta. 2015 Jan;132:1-7. doi: 10.1016/j.talanta.2014.08.047. Epub 2014 Aug 27.