PMID- 25476401
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150807
LR  - 20141205
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 132
DP  - 2015 Jan
TI  - Retention behavior of microparticles in gravitational field-flow fractionation 
      (GrFFF): effect of ionic strength.
PG  - 945-53
LID - S0039-9140(14)00457-3 [pii]
LID - 10.1016/j.talanta.2014.05.061 [doi]
AB  - Retention behavior of micron-sized particles in gravitational field-flow 
      fractionation (GrFFF) was studied in this study. Effects of ionic strength and 
      flow rate as well as the viscosity of the GrFFF carrier liquid was investigated 
      on the size-based selectivity (Sd), retention ratio (R), and plate height (H) of 
      micron-sized particles using polystyrene latex beads as model particles. It was 
      found that the retention ratio of microparticles increases with increasing flow 
      rate or the viscosity of the carrier liquid as the particles are forced away from 
      the accumulation wall by increased hydrodynamic lift forces (HLF). On the other 
      hand, the retention time increases (retention ratio decreases) with increasing 
      ionic strength of the carrier liquid at the same flow rate, due to decreased 
      repulsive interaction between the particles and the channel accumulation wall 
      (glass in this study) allowing the particles approach closer to the wall. Results 
      suggest the ionic strength of the carrier liquid plays a critical role in 
      determining retention of microparticles in GrFFF as well as the viscosity or the 
      flow rate of the carrier liquid. It was found that the resolution and the 
      separation time could be improved by increasing the carrier viscosity and by 
      carefully adjusting the ionic strength of the carrier liquid.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Woo, In Suk
AU  - Woo IS
AD  - Department of Chemistry, Hannam University, Daejeon 305-811, Korea Republic.
FAU - Jung, Euo Chang
AU  - Jung EC
AD  - Nuclear Chemistry Research Center, Korea Atomic Energy Research Institute, 
      Daejeon 305-353, Korea Republic.
FAU - Lee, Seungho
AU  - Lee S
AD  - Department of Chemistry, Hannam University, Daejeon 305-811, Korea Republic. 
      Electronic address: slee@hannam.kr.
LA  - eng
PT  - Journal Article
DEP - 20140610
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
OTO - NOTNLM
OT  - Gravitational field-flow fractionation (GrFFF)
OT  - Ionic strength
OT  - Microparticles
OT  - Resolution
OT  - Viscosity
EDAT- 2014/12/06 06:00
MHDA- 2014/12/06 06:01
CRDT- 2014/12/06 06:00
PHST- 2014/04/27 00:00 [received]
PHST- 2014/05/28 00:00 [revised]
PHST- 2014/05/29 00:00 [accepted]
PHST- 2014/12/06 06:00 [entrez]
PHST- 2014/12/06 06:00 [pubmed]
PHST- 2014/12/06 06:01 [medline]
AID - S0039-9140(14)00457-3 [pii]
AID - 10.1016/j.talanta.2014.05.061 [doi]
PST - ppublish
SO  - Talanta. 2015 Jan;132:945-53. doi: 10.1016/j.talanta.2014.05.061. Epub 2014 Jun 
      10.