PMID- 25476687
OWN - NLM
STAT- MEDLINE
DCOM- 20150818
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1374
DP  - 2014 Dec 29
TI  - Liquid chromatography-electrospray-tandem mass spectrometry method for 
      determination of organophosphate diesters in biotic samples including Great Lakes 
      herring gull plasma.
PG  - 85-92
LID - S0021-9673(14)01780-4 [pii]
LID - 10.1016/j.chroma.2014.11.022 [doi]
AB  - Environmentally relevant organophosphate (OP) triester flame retardants are known 
      to degrade to OP diester phosphoric acids. In this study, a quantitatively 
      sensitive method was developed for OP diesters in biological samples of varying 
      complexity, bovine serum, chicken egg homogenate and pork liver. Fortified with 
      1ng or 10ng each of the six OP diester and six OP triester standards, samples 
      were extracted by accelerated solvent extraction that completely separated OP 
      diesters and triesters. OP diester fractions were cleaned up using weak anion 
      exchange solid phase extraction and eluted with high ionic strength ammonium 
      acetate buffer. Optimal analysis of chlorinated OP diesters was via decamethonium 
      hydroxide dicationic reagent derivatization and by LC-ESI(+)-MS/MS, and for all 
      non-chlorinated OP diesters by non-derivatized LC-ESI(-)-MS/MS. Except for 
      derivatization LC-ESI(+)-MS/MS analysis of liver, at the 10ng spiking level for 
      the three matrices, recovery efficiencies, matrix effects and method limits of 
      quantification (MLOQs) of OP diesters ranged from 55-116%, 92-119%, and 
      0.02-0.31ng/g wet weight (ww) respectively. Plasma samples of n=6 herring gulls 
      (2010, Chantry Is., Laurentian Great Lakes) contained triphenyl phosphate and 
      tris(1-3-dichloro-2-propyl) phosphate ranging from 1.3 to 4.0ng/g ww and <MLOQ to 
      0.41ng/g ww respectively. The OP diesters bis(1,3-dichloro-2-propyl) phosphate, 
      bis-(2-butoxyethyl) phosphate and di(2-ethylhexyl) phosphate ranged from 0.7 to 
      3.5ng/g ww, 0.08 to 29.4ng/g ww and <MLOQ to 0.18ng/g ww respectively, and thus 
      OP triester to diester metabolism occurs in exposed wild herring gulls.
CI  - Crown Copyright (c) 2014. Published by Elsevier B.V. All rights reserved.
FAU - Su, Guanyong
AU  - Su G
AD  - Ecotoxicology and Wildlife Heath Division, Environment Canada, National Wildlife 
      Research Centre, Carleton University, Ottawa, ON K1A 0H3, Canada; Department of 
      Chemistry, Carleton University, Ottawa, ON K1S 5B6, Canada.
FAU - Greaves, Alana K
AU  - Greaves AK
AD  - Ecotoxicology and Wildlife Heath Division, Environment Canada, National Wildlife 
      Research Centre, Carleton University, Ottawa, ON K1A 0H3, Canada; Department of 
      Chemistry, Carleton University, Ottawa, ON K1S 5B6, Canada.
FAU - Gauthier, Lewis
AU  - Gauthier L
AD  - Ecotoxicology and Wildlife Heath Division, Environment Canada, National Wildlife 
      Research Centre, Carleton University, Ottawa, ON K1A 0H3, Canada.
FAU - Letcher, Robert J
AU  - Letcher RJ
AD  - Ecotoxicology and Wildlife Heath Division, Environment Canada, National Wildlife 
      Research Centre, Carleton University, Ottawa, ON K1A 0H3, Canada; Department of 
      Chemistry, Carleton University, Ottawa, ON K1S 5B6, Canada. Electronic address: 
      Robert.Letcher@ec.gc.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141126
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Esters)
RN  - 0 (Organophosphates)
RN  - 126-72-7 (tris(2,3-dibromopropyl)phosphate)
RN  - YZE19Z66EA (triphenyl phosphate)
SB  - IM
MH  - Animals
MH  - Charadriiformes/*blood
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Esters/*blood
MH  - Lakes
MH  - Organophosphates/*blood
MH  - Solid Phase Extraction/methods
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Egg
OT  - Great Lakes herring gulls
OT  - LC-ESI-MS/MS
OT  - Liver
OT  - Organophosphate flame retardants and diesters
OT  - Serum
EDAT- 2014/12/06 06:00
MHDA- 2015/08/19 06:00
CRDT- 2014/12/06 06:00
PHST- 2014/07/09 00:00 [received]
PHST- 2014/11/07 00:00 [revised]
PHST- 2014/11/10 00:00 [accepted]
PHST- 2014/12/06 06:00 [entrez]
PHST- 2014/12/06 06:00 [pubmed]
PHST- 2015/08/19 06:00 [medline]
AID - S0021-9673(14)01780-4 [pii]
AID - 10.1016/j.chroma.2014.11.022 [doi]
PST - ppublish
SO  - J Chromatogr A. 2014 Dec 29;1374:85-92. doi: 10.1016/j.chroma.2014.11.022. Epub 
      2014 Nov 26.