PMID- 25476802
OWN - NLM
STAT- MEDLINE
DCOM- 20151109
LR  - 20181202
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 5
IP  - 6
DP  - 2014 Dec 4
TI  - Human mesenchymal stem cells possess different biological characteristics but do 
      not change their therapeutic potential when cultured in serum free medium.
PG  - 132
LID - 10.1186/scrt522 [doi]
LID - 132
AB  - INTRODUCTION: Mesenchymal stem cells (MSCs) are widely investigated in clinical 
      researches to treat various diseases. Classic culture medium for MSCs, even for 
      clinical use, contains fetal bovine serum. The serum-containing medium (SCM) 
      seems a major obstacle for MSCs-related therapies due to the risk of 
      contamination of infectious pathogens. Some studies showed that MSCs could be 
      expanded in serum free medium (SFM); however, whether SFM would change the 
      biological characteristics and safety issues of MSCs has not been well answered. 
      METHODS: Human umbilical cord mesenchymal stem cells (hUC-MSCs) were cultured in 
      a chemical defined serum free medium. Growth, multipotency, surface antigen 
      expression, telomerase, immunosuppressive ability, gene expression profile and 
      genomic stability of hUC-MSCs cultured in SFM and SCM were analyzed and compared 
      side by side. RESULTS: hUC-MSCs propagated more slowly and senesce ultimately in 
      SFM. SFM-expanded hUC-MSCs were different from SCM-expanded hUC-MSCs in growth 
      rate, telomerase, gene expression profile. However, SFM-expanded hUC-MSCs 
      maintained multipotency and the profile of surface antigen which were used to 
      define human MSCs. Both SFM- and SCM-expanded hUC-MSCs gained copy number 
      variation (CNV) in long-term in vitro culture. CONCLUSION: hUC-MCSs could be 
      expanded in SFM safely to obtain enough cells for clinical application, meeting 
      the basic criteria for human mesenchymal stem cells. hUC-MSCs cultured in SFM 
      were distinct from hUC-MSCs cultured in SCM, yet they remained therapeutic 
      potentials for future regenerative medicine.
FAU - Wang, Youwei
AU  - Wang Y
FAU - Wu, Hehe
AU  - Wu H
FAU - Yang, Zhouxin
AU  - Yang Z
FAU - Chi, Ying
AU  - Chi Y
FAU - Meng, Lei
AU  - Meng L
FAU - Mao, Aibin
AU  - Mao A
FAU - Yan, Shulin
AU  - Yan S
FAU - Hu, Shanshan
AU  - Hu S
FAU - Zhang, Jianzhong
AU  - Zhang J
FAU - Zhang, Yun
AU  - Zhang Y
FAU - Yu, Wenbo
AU  - Yu W
FAU - Ma, Yue
AU  - Ma Y
FAU - Li, Tao
AU  - Li T
FAU - Cheng, Yan
AU  - Cheng Y
FAU - Wang, Yongjuan
AU  - Wang Y
FAU - Wang, Shanshan
AU  - Wang S
FAU - Liu, Jing
AU  - Liu J
FAU - Han, Jingwen
AU  - Han J
FAU - Li, Caiyun
AU  - Li C
FAU - Liu, Li
AU  - Liu L
FAU - Xu, Jian
AU  - Xu J
FAU - Han, Zhi Bo
AU  - Han ZB
FAU - Han, Zhong Chao
AU  - Han ZC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141204
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - 0 (Culture Media, Serum-Free)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Culture Media, Serum-Free/pharmacology
MH  - DNA Copy Number Variations
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/drug effects/metabolism
MH  - Telomerase/genetics/metabolism
PMC - PMC4445567
EDAT- 2014/12/06 06:00
MHDA- 2015/11/10 06:00
PMCR- 2014/12/04
CRDT- 2014/12/06 06:00
PHST- 2014/02/19 00:00 [received]
PHST- 2014/11/19 00:00 [accepted]
PHST- 2014/12/06 06:00 [entrez]
PHST- 2014/12/06 06:00 [pubmed]
PHST- 2015/11/10 06:00 [medline]
PHST- 2014/12/04 00:00 [pmc-release]
AID - scrt522 [pii]
AID - 442 [pii]
AID - 10.1186/scrt522 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2014 Dec 4;5(6):132. doi: 10.1186/scrt522.