PMID- 25477779
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141205
LR  - 20220309
IS  - 1662-5099 (Print)
IS  - 1662-5099 (Electronic)
IS  - 1662-5099 (Linking)
VI  - 7
DP  - 2014
TI  - Putting a finishing touch on GECIs.
PG  - 88
LID - 10.3389/fnmol.2014.00088 [doi]
LID - 88
AB  - More than a decade ago genetically encoded calcium indicators (GECIs) entered the 
      stage as new promising tools to image calcium dynamics and neuronal activity in 
      living tissues and designated cell types in vivo. From a variety of initial 
      designs two have emerged as promising prototypes for further optimization: FRET 
      (Forster Resonance Energy Transfer)-based sensors and single fluorophore sensors 
      of the GCaMP family. Recent efforts in structural analysis, engineering and 
      screening have broken important performance thresholds in the latest generation 
      for both classes. While these improvements have made GECIs a powerful means to 
      perform physiology in living animals, a number of other aspects of sensor 
      function deserve attention. These aspects include indicator linearity, toxicity 
      and slow response kinetics. Furthermore creating high performance sensors with 
      optically more favorable emission in red or infrared wavelengths as well as new 
      stably or conditionally GECI-expressing animal lines are on the wish list. When 
      the remaining issues are solved, imaging of GECIs will finally have crossed the 
      last milestone, evolving from an initial promise into a fully matured technology.
FAU - Rose, Tobias
AU  - Rose T
AD  - Max-Planck-Institute of Neurobiology Martinsried, Germany.
FAU - Goltstein, Pieter M
AU  - Goltstein PM
AD  - Max-Planck-Institute of Neurobiology Martinsried, Germany.
FAU - Portugues, Ruben
AU  - Portugues R
AD  - Max-Planck-Institute of Neurobiology Martinsried, Germany.
FAU - Griesbeck, Oliver
AU  - Griesbeck O
AD  - Max-Planck-Institute of Neurobiology Martinsried, Germany.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20141118
PL  - Switzerland
TA  - Front Mol Neurosci
JT  - Frontiers in molecular neuroscience
JID - 101477914
PMC - PMC4235368
OTO - NOTNLM
OT  - FRET
OT  - buffering
OT  - calcium
OT  - fluorescent protein
OT  - imaging
OT  - neuronal activity
OT  - segmentation
EDAT- 2014/12/06 06:00
MHDA- 2014/12/06 06:01
PMCR- 2014/01/01
CRDT- 2014/12/06 06:00
PHST- 2014/08/19 00:00 [received]
PHST- 2014/10/29 00:00 [accepted]
PHST- 2014/12/06 06:00 [entrez]
PHST- 2014/12/06 06:00 [pubmed]
PHST- 2014/12/06 06:01 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 10.3389/fnmol.2014.00088 [doi]
PST - epublish
SO  - Front Mol Neurosci. 2014 Nov 18;7:88. doi: 10.3389/fnmol.2014.00088. eCollection 
      2014.