PMID- 25478818 OWN - NLM STAT- MEDLINE DCOM- 20150826 LR - 20161020 IS - 1480-3321 (Electronic) IS - 0831-2796 (Linking) VI - 57 IP - 9 DP - 2014 Sep TI - Exploring the origin of the D genome of oat by fluorescence in situ hybridization. PG - 469-72 LID - 10.1139/gen-2014-0048 [doi] AB - Further understanding of the origin of cultivated oat would accelerate its genetic improvement. In particular, it would be useful to clarify which diploid progenitor contributed the D genome of this allohexaploid species. In this study, we demonstrate that the landmarks produced by fluorescence in situ hybridization (FISH) of species of Avena using probes derived from Avena sativa can be used to explore the origin of the D genome. Selected sets of probes were hybridized in several sequential experiments performed on exactly the same chromosome spreads, with multiple probes of cytological preparations. Probes pITS and A3-19 showed there might be a similar distribution of pITS between the Ac and D genomes. These results indicated that the Ac genome is closely related to the D genome, and that Avena canariensis (AcAc) could be the D-genome donor of cultivated oat. FAU - Luo, Xiaomei AU - Luo X AD - a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China. FAU - Zhang, Haiqin AU - Zhang H FAU - Kang, Houyang AU - Kang H FAU - Fan, Xing AU - Fan X FAU - Wang, Yi AU - Wang Y FAU - Sha, Lina AU - Sha L FAU - Zhou, Yonghong AU - Zhou Y LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141014 PL - Canada TA - Genome JT - Genome JID - 8704544 SB - IM MH - Avena/*genetics MH - *Genome, Plant MH - In Situ Hybridization, Fluorescence MH - Metaphase OTO - NOTNLM OT - Avena canariensis OT - D genome OT - FISH OT - avoine hexaploide OT - genome D OT - hexaploid oat OT - origin OT - origine EDAT- 2014/12/06 06:00 MHDA- 2015/08/27 06:00 CRDT- 2014/12/06 06:00 PHST- 2014/12/06 06:00 [entrez] PHST- 2014/12/06 06:00 [pubmed] PHST- 2015/08/27 06:00 [medline] AID - 10.1139/gen-2014-0048 [doi] PST - ppublish SO - Genome. 2014 Sep;57(9):469-72. doi: 10.1139/gen-2014-0048. Epub 2014 Oct 14.