PMID- 25479718
OWN - NLM
STAT- MEDLINE
DCOM- 20150914
LR  - 20220408
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 24
IP  - 1
DP  - 2015 Jan
TI  - Berberine hydrochloride attenuates lipopolysaccharide-induced endometritis in 
      mice by suppressing activation of NF-kappaB signal pathway.
PG  - 128-32
LID - S1567-5769(14)00437-8 [pii]
LID - 10.1016/j.intimp.2014.11.002 [doi]
AB  - Endometritis is a common disease in animal production and influences breeding all 
      over the world. Berberine is one of the main alkaloids isolated from Rhizoma 
      coptidis. Previous reports showed that berberine has anti-inflammatory potential. 
      However, there have been a limited number of published reports on the 
      anti-inflammatory effect of berberine hydrochloride on LPS-induced endometritis. 
      The purpose of the present study was to investigate the effects of berberine 
      hydrochloride on LPS-induced mouse endometritis. Berberine hydrochloride was 
      administered intraperitoneally at 1h before and 12h after LPS induction. Then, a 
      biopsy was performed, and uterine myeloperoxidase (MPO) and nitric oxide (NO) 
      concentrations were determined. Tumor necrosis factor-alpha (TNF-alpha) and 
      interleukin-1beta (IL-1beta) levels in the uterus homogenate were measured by ELISA. 
      The extent of IkappaB-alpha and P65 phosphorylation was detected by Western blot. The 
      results showed that berberine hydrochloride significantly attenuated neutrophil 
      infiltration, suppressed myeloperoxidase activity and decreased NO, TNF-alphaand 
      IL-1betaproduction. Furthermore, berberine hydrochloride inhibited the 
      phosphorylation of the NF-kappaB p65 subunit and the degradation of its inhibitor, 
      IkappaBalpha. These findings suggest that berberine hydrochloride exerts potent 
      anti-inflammatory effects on LPS-induced mouse endometritis and might be a 
      potential therapeutic agent for endometritis.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Fu, Kaiqiang
AU  - Fu K
AD  - Department of Clinical Veterinary Medicine, College of Animal Science and 
      Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 
      266109, People's Republic of China.
FAU - Lv, Xiaopei
AU  - Lv X
AD  - Department of Clinical Veterinary Medicine, College of Animal Science and 
      Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 
      266109, People's Republic of China.
FAU - Li, Weishi
AU  - Li W
AD  - Department of Clinical Veterinary Medicine, College of Animal Science and 
      Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 
      266109, People's Republic of China.
FAU - Wang, Yu
AU  - Wang Y
AD  - Department of Clinical Veterinary Medicine, College of Animal Science and 
      Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 
      266109, People's Republic of China.
FAU - Li, Huatao
AU  - Li H
AD  - Department of Clinical Veterinary Medicine, College of Animal Science and 
      Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 
      266109, People's Republic of China.
FAU - Tian, Wenru
AU  - Tian W
AD  - Department of Clinical Veterinary Medicine, College of Animal Science and 
      Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 
      266109, People's Republic of China.
FAU - Cao, Rongfeng
AU  - Cao R
AD  - Department of Clinical Veterinary Medicine, College of Animal Science and 
      Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 
      266109, People's Republic of China. Electronic address: rfcao@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141115
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Cytokines)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Nfkbia protein, mouse)
RN  - 0I8Y3P32UF (Berberine)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*administration & dosage
MH  - Berberine/*administration & dosage
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Endometritis/chemically induced/*drug therapy/immunology
MH  - Female
MH  - I-kappa B Proteins/metabolism
MH  - Inflammation Mediators/metabolism
MH  - Lipopolysaccharides/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - NF-KappaB Inhibitor alpha
MH  - Neutrophils/*drug effects/immunology
MH  - Nitric Oxide/metabolism
MH  - Peroxidase/metabolism
MH  - Phosphorylation/drug effects
MH  - Pinellia/immunology
MH  - Signal Transduction/drug effects
MH  - Uterus/*drug effects/metabolism
OTO - NOTNLM
OT  - Berberine hydrochloride
OT  - Endometritis
OT  - LPS
OT  - NF-kappaB
EDAT- 2014/12/07 06:00
MHDA- 2015/09/15 06:00
CRDT- 2014/12/07 06:00
PHST- 2014/09/03 00:00 [received]
PHST- 2014/11/05 00:00 [revised]
PHST- 2014/11/05 00:00 [accepted]
PHST- 2014/12/07 06:00 [entrez]
PHST- 2014/12/07 06:00 [pubmed]
PHST- 2015/09/15 06:00 [medline]
AID - S1567-5769(14)00437-8 [pii]
AID - 10.1016/j.intimp.2014.11.002 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2015 Jan;24(1):128-32. doi: 10.1016/j.intimp.2014.11.002. 
      Epub 2014 Nov 15.