PMID- 25481230
OWN - NLM
STAT- MEDLINE
DCOM- 20150918
LR  - 20151119
IS  - 1878-5875 (Electronic)
IS  - 1357-2725 (Linking)
VI  - 59
DP  - 2015 Feb
TI  - Involvement of BK channel in differentiation of vascular smooth muscle cells 
      induced by mechanical stretch.
PG  - 21-9
LID - S1357-2725(14)00376-8 [pii]
LID - 10.1016/j.biocel.2014.11.011 [doi]
AB  - The differentiation of vascular smooth muscle cells (VSMCs), which are exposed to 
      mechanical stretch in vivo, plays an important role in vascular remodeling during 
      hypertension. Here, we demonstrated the mechanobiological roles of large 
      conductance calcium and voltage-activated potassium (BK) channels in this 
      process. In comparison with 5% stretch (physiological), 15% stretch 
      (pathological) induced the de-differentiation of VSMCs, resulting in 
      significantly decreased expressions of VSMC markers, i.e., alpha-actin, calponin and 
      SM22. The activity of BK channels, assessed by patch clamp recording, was 
      significantly increased by 15% stretch and was accompanied by an increased 
      alternative splicing of BK channel alpha-subunit at the stress axis-regulated exons 
      (STREX). Furthermore, transfection of whole BK or STREX-deleted BK plasmids 
      revealed that STREX was important for BK channels to sense mechanical stretch. 
      Using thapsigargin (TG) which induces endoplasmic reticulum (ER) stress, and 
      xbp1-targeted siRNA transfection which blocks ER stress, the results revealed 
      that ER stress was contribute to stretch-induced alternative splicing of STREX. 
      Our results suggested that during hypertension, pathological stretch may induce 
      the ER stress in VSMCs, which affects the alternative splicing and activity of BK 
      channels, and subsequently modulates VSMC differentiation.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Wan, Xue-Jiao
AU  - Wan XJ
AD  - Institute of Mechanobiology & Medical Engineering, School of Life Sciences & 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Zhao, Hu-Cheng
AU  - Zhao HC
AD  - Lab of Biomechanics, Department of Engineering Mechanics, Tsinghua University, 
      Beijing, China.
FAU - Zhang, Ping
AU  - Zhang P
AD  - Institute of Mechanobiology & Medical Engineering, School of Life Sciences & 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Huo, Bo
AU  - Huo B
AD  - School of Aerospace Engineering, Beijing Institute of Technology, Beijing, China.
FAU - Shen, Bao-Rong
AU  - Shen BR
AD  - Institute of Mechanobiology & Medical Engineering, School of Life Sciences & 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Yan, Zhi-Qiang
AU  - Yan ZQ
AD  - Institute of Mechanobiology & Medical Engineering, School of Life Sciences & 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Qi, Ying-Xin
AU  - Qi YX
AD  - Institute of Mechanobiology & Medical Engineering, School of Life Sciences & 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China. Electronic 
      address: qiyx@sjtu.edu.cn.
FAU - Jiang, Zong-Lai
AU  - Jiang ZL
AD  - Institute of Mechanobiology & Medical Engineering, School of Life Sciences & 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141204
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (Biomarkers)
RN  - 0 (Large-Conductance Calcium-Activated Potassium Channels)
RN  - 0 (Protein Subunits)
RN  - 67526-95-8 (Thapsigargin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alternative Splicing/drug effects/genetics
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Calcium/metabolism
MH  - *Cell Differentiation/drug effects
MH  - Endoplasmic Reticulum Stress/drug effects
MH  - Exons/genetics
MH  - HEK293 Cells
MH  - Humans
MH  - Ion Channel Gating/drug effects
MH  - Large-Conductance Calcium-Activated Potassium Channels/*metabolism
MH  - Muscle, Smooth, Vascular/*cytology
MH  - Myocytes, Smooth Muscle/*cytology/drug effects/*metabolism
MH  - Protein Subunits/metabolism
MH  - Rats, Sprague-Dawley
MH  - *Stress, Mechanical
MH  - Thapsigargin/pharmacology
OTO - NOTNLM
OT  - BK channel
OT  - Differentiation
OT  - Endoplasmic reticulum stress
OT  - Mechanical stretch
OT  - Vascular smooth muscle cells
EDAT- 2014/12/08 06:00
MHDA- 2015/09/19 06:00
CRDT- 2014/12/08 06:00
PHST- 2014/08/07 00:00 [received]
PHST- 2014/11/13 00:00 [revised]
PHST- 2014/11/25 00:00 [accepted]
PHST- 2014/12/08 06:00 [entrez]
PHST- 2014/12/08 06:00 [pubmed]
PHST- 2015/09/19 06:00 [medline]
AID - S1357-2725(14)00376-8 [pii]
AID - 10.1016/j.biocel.2014.11.011 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 2015 Feb;59:21-9. doi: 10.1016/j.biocel.2014.11.011. 
      Epub 2014 Dec 4.