PMID- 25483313
OWN - NLM
STAT- MEDLINE
DCOM- 20150918
LR  - 20150120
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Linking)
VI  - 26
IP  - 2
DP  - 2015 Feb
TI  - Skeletal muscle Sirt3 expression and mitochondrial respiration are regulated by a 
      prenatal low-protein diet.
PG  - 184-9
LID - S0955-2863(14)00221-6 [pii]
LID - 10.1016/j.jnutbio.2014.10.003 [doi]
AB  - Malnutrition during the fetal growth period increases risk for later obesity and 
      type 2 diabetes mellitus (T2DM). We have shown that a prenatal low-protein (8% 
      protein; LP) diet followed by postnatal high-fat (45% fat; HF) diet results in 
      offspring propensity for adipose tissue catch-up growth, obesity and T2DM in 
      Sprague-Dawley rats. Skeletal muscle is the major tissue for insulin-mediated 
      glucose uptake. Dysfunctional skeletal muscle mitochondrial function, 
      particularly reduction in expression of mitochondrial protein sirtuin protein 3 
      (Sirt3) contributes to development of T2DM by reducing mitochondrial respiration. 
      Therefore, we hypothesized that maternal LP and postnatal HF diets would increase 
      T2DM risk due to Sirt3 dysfunction within skeletal muscle mitochondria. Using our 
      maternal LP and postnatal HF diet model, we showed that skeletal muscle 
      mitochondrial oxygen consumption rate was decreased by maternal LP diet. 
      Mitochondria copy number, mitochondrial thermogenesis (UCP-1) expression and 
      mitochondrial biogenic factors including nuclear respiratory factor 1 and 
      cytochrome c oxidases 1 and 4 were unaffected by maternal LP and postnatal HF 
      diets. Skeletal muscle Sirt3 mRNA decreased with maternal LP diet. A 
      mitochondrial substrate of Sirt3, succinate dehydrogenase (SDH), is regulated by 
      Sirt3 via lysine residue acetylation status of SDH. Acetylated SDH protein 
      (inactive form) levels were moderately decreased by maternal LP diet. Taken 
      together, these data suggest that maternal LP and postnatal HF diets may increase 
      the risk for T2D by decreasing skeletal muscle oxidative respiration via 
      increased Sirt3 and possibly by decreased amounts of the active form of SDH 
      enzyme.
CI  - Published by Elsevier Inc.
FAU - Claycombe, Kate J
AU  - Claycombe KJ
AD  - USDA-ARS, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58203, 
      USA. Electronic address: kate.claycombe@ars.usda.gov.
FAU - Roemmich, James N
AU  - Roemmich JN
AD  - USDA-ARS, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58203, 
      USA.
FAU - Johnson, LuAnn
AU  - Johnson L
AD  - USDA-ARS, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58203, 
      USA.
FAU - Vomhof-DeKrey, Emilie E
AU  - Vomhof-DeKrey EE
AD  - USDA-ARS, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58203, 
      USA.
FAU - Johnson, W Thomas
AU  - Johnson WT
AD  - USDA-ARS, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58203, 
      USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20141114
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Protein Subunits)
RN  - EC 1.3.99.1 (Succinate Dehydrogenase)
RN  - EC 3.5.1.- (Sirtuin 3)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Animals, Newborn
MH  - Diabetes Mellitus, Type 2/etiology/metabolism/pathology
MH  - Diet, High-Fat/adverse effects
MH  - Diet, Protein-Restricted/*adverse effects
MH  - Disease Susceptibility
MH  - Down-Regulation
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Male
MH  - *Maternal Nutritional Physiological Phenomena
MH  - Mitochondria, Muscle/enzymology/*metabolism/pathology
MH  - Muscle, Skeletal/growth & development/*metabolism/pathology
MH  - Pregnancy
MH  - Protein Processing, Post-Translational
MH  - Protein Subunits/metabolism
MH  - Rats, Sprague-Dawley
MH  - Sirtuin 3/genetics/*metabolism
MH  - Succinate Dehydrogenase/*metabolism
OTO - NOTNLM
OT  - Maternal low-protein diet
OT  - Mitochondria
OT  - SDH
OT  - SIRT3
OT  - Skeletal muscle respiration
EDAT- 2014/12/09 06:00
MHDA- 2015/09/19 06:00
CRDT- 2014/12/09 06:00
PHST- 2014/06/20 00:00 [received]
PHST- 2014/10/01 00:00 [revised]
PHST- 2014/10/02 00:00 [accepted]
PHST- 2014/12/09 06:00 [entrez]
PHST- 2014/12/09 06:00 [pubmed]
PHST- 2015/09/19 06:00 [medline]
AID - S0955-2863(14)00221-6 [pii]
AID - 10.1016/j.jnutbio.2014.10.003 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2015 Feb;26(2):184-9. doi: 10.1016/j.jnutbio.2014.10.003. Epub 
      2014 Nov 14.