PMID- 25483331
OWN - NLM
STAT- MEDLINE
DCOM- 20150903
LR  - 20220330
IS  - 1949-0984 (Electronic)
IS  - 1949-0976 (Print)
IS  - 1949-0976 (Linking)
VI  - 5
IP  - 5
DP  - 2014
TI  - Zinc deficiency alters host response and pathogen virulence in a mouse model of 
      enteroaggregative Escherichia coli-induced diarrhea.
PG  - 618-27
LID - 10.4161/19490976.2014.969642 [doi]
AB  - Enteroaggregative Escherichia coli (EAEC) is increasingly recognized as a major 
      cause of diarrheal disease globally. In the current study, we investigated the 
      impact of zinc deficiency on the host and pathogenesis of EAEC. Several outcomes 
      of EAEC infection were investigated including weight loss, EAEC shedding and 
      tissue burden, leukocyte recruitment, intestinal cytokine expression, and 
      virulence expression of the pathogen in vivo. Mice fed a protein source defined 
      zinc deficient diet (dZD) had an 80% reduction of serum zinc and a 50% reduction 
      of zinc in luminal contents of the bowel compared to mice fed a protein source 
      defined control diet (dC). When challenged with EAEC, dZD mice had significantly 
      greater weight loss, stool shedding, mucus production, and, most notably, 
      diarrhea compared to dC mice. Zinc deficient mice had reduced infiltration of 
      leukocytes into the ileum in response to infection suggesting an impaired immune 
      response. Interestingly, expression of several EAEC virulence factors were 
      increased in luminal contents of dZD mice. These data show a dual effect of 
      dietary zinc in benefitting the host while impairing virulence of the pathogen. 
      The study demonstrates the critical importance of zinc and may help elucidate the 
      benefits of zinc supplementation in cases of childhood diarrhea and malnutrition.
FAU - Bolick, David T
AU  - Bolick DT
AD  - a Center for Global Health ; Division of Infectious Diseases and International 
      Health ; School of Medicine ; University of Virginia ; Charlottesville , VA USA.
FAU - Kolling, Glynis L
AU  - Kolling GL
FAU - Moore, John H 2nd
AU  - Moore JH 2nd
FAU - de Oliveira, Luis Antonio
AU  - de Oliveira LA
FAU - Tung, Kenneth
AU  - Tung K
FAU - Philipson, Casandra
AU  - Philipson C
FAU - Viladomiu, Monica
AU  - Viladomiu M
FAU - Hontecillas, Raquel
AU  - Hontecillas R
FAU - Bassaganya-Riera, Josep
AU  - Bassaganya-Riera J
FAU - Guerrant, Richard L
AU  - Guerrant RL
LA  - eng
GR  - D43 TW006578/TW/FIC NIH HHS/United States
GR  - D43TW006578/TW/FIC NIH HHS/United States
GR  - HHSN272201000056C/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Gut Microbes
JT  - Gut microbes
JID - 101495343
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Bacterial Shedding
MH  - Body Weight
MH  - Diarrhea/*immunology/*microbiology/pathology
MH  - Disease Models, Animal
MH  - Disease Susceptibility
MH  - Escherichia coli/*isolation & purification
MH  - Escherichia coli Infections/*immunology/*microbiology/pathology
MH  - Ileum/pathology
MH  - Leukocytes/immunology
MH  - Male
MH  - Mice
MH  - Zinc/*deficiency
PMC - PMC4615194
OTO - NOTNLM
OT  - Cftr, cystic fibrosis transmembrane regulator
OT  - EAEC, Enteroaggregative Escherichia coli
OT  - Enteroaggregative Escherichia coli
OT  - dC, defined control diet
OT  - dZD, defined zinc deficient diet
OT  - diarrhea
OT  - enteropathy
OT  - virulence
OT  - zinc deficiency
EDAT- 2014/12/09 06:00
MHDA- 2015/09/04 06:00
PMCR- 2015/10/30
CRDT- 2014/12/09 06:00
PHST- 2014/12/09 06:00 [entrez]
PHST- 2014/12/09 06:00 [pubmed]
PHST- 2015/09/04 06:00 [medline]
PHST- 2015/10/30 00:00 [pmc-release]
AID - 969642 [pii]
AID - 10.4161/19490976.2014.969642 [doi]
PST - ppublish
SO  - Gut Microbes. 2014;5(5):618-27. doi: 10.4161/19490976.2014.969642.