PMID- 25483846
OWN - NLM
STAT- MEDLINE
DCOM- 20150821
LR  - 20150112
IS  - 1096-0805 (Electronic)
IS  - 0022-2011 (Linking)
VI  - 124
DP  - 2015 Jan
TI  - Emerging and endemic types of Ostreid herpesvirus 1 were detected in bivalves in 
      China.
PG  - 98-106
LID - S0022-2011(14)00178-5 [pii]
LID - 10.1016/j.jip.2014.11.007 [doi]
AB  - Viral infection caused by Ostreid herpesvirus 1 (OsHV-1) is one of the proximate 
      causes of mass mortalities of cultivated bivalves around the world. The emergence 
      and spread of different variants of OsHV-1 accompanied by different 
      epidemiological characteristics have been reported frequently in different 
      countries around the world. In this paper, we present a study of the detection of 
      OsHV-1 DNA and their variations from 1599 samples over 18 species collected in 27 
      aquaculture sites and two food markets during 2001-2013 in China. All of the 
      samples were examined by a nested PCR assay targeting the C2/C6 fragment of 
      OsHV-1 followed by sequencing. Our results showed 338 individuals (21.1%) of 
      seven species sampled from 14 (14/27=51.9%) sites and the two food markets were 
      positive for viral DNA. Sequencing of 289 PCR products revealed 24 virus types. 
      No shared virus type was found among different countries with 47 types (23 in 
      Japan, 16 in France, 2 in South Korea and 1 in each country of Australia, USA, 
      Ireland, New Zealand, Mexico and China) identified in previous studies. As 
      previously reported, two main phylogenetic groups were identified by phylogenetic 
      analysis based on the 71 virus types; within which 6 separate clades were 
      identified. Our results also demonstrated that two clades were associated with 
      abnormal mortalities of the scallop, Chlamys farrier and the calm, Scapharca 
      broughtonii in China. These findings indicated that cultivated bivalves may face 
      potential threats from OsHV-1 types found in our study.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Bai, Changming
AU  - Bai C
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao, China; Function Laboratory for Marine Fisheries Science and 
      Food Production Processes, Qingdao National Oceanography Laboratory, Qingdao, 
      China.
FAU - Wang, Chongming
AU  - Wang C
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao, China; Function Laboratory for Marine Fisheries Science and 
      Food Production Processes, Qingdao National Oceanography Laboratory, Qingdao, 
      China. Electronic address: wangcm@ysfri.ac.cn.
FAU - Xia, Junyang
AU  - Xia J
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao, China.
FAU - Sun, Hailin
AU  - Sun H
AD  - Changdao County Oceanic and Fishery Administration, Yantai, China.
FAU - Zhang, Shuai
AU  - Zhang S
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao, China.
FAU - Huang, Jie
AU  - Huang J
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao, China; Function Laboratory for Marine Fisheries Science and 
      Food Production Processes, Qingdao National Oceanography Laboratory, Qingdao, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141205
PL  - United States
TA  - J Invertebr Pathol
JT  - Journal of invertebrate pathology
JID - 0014067
SB  - IM
MH  - Animals
MH  - China
MH  - Crassostrea/*virology
MH  - DNA Viruses/classification/*isolation & purification
MH  - Environmental Monitoring
MH  - Pectinidae/*virology
MH  - Phylogeny
OTO - NOTNLM
OT  - Chlamys farreri
OT  - Crassostrea gigas
OT  - Mass mortality
OT  - Molecular epidemiology
OT  - OsHV-1
OT  - Ostreid herpesvirus 1
EDAT- 2014/12/09 06:00
MHDA- 2015/08/22 06:00
CRDT- 2014/12/09 06:00
PHST- 2014/08/18 00:00 [received]
PHST- 2014/11/17 00:00 [revised]
PHST- 2014/11/25 00:00 [accepted]
PHST- 2014/12/09 06:00 [entrez]
PHST- 2014/12/09 06:00 [pubmed]
PHST- 2015/08/22 06:00 [medline]
AID - S0022-2011(14)00178-5 [pii]
AID - 10.1016/j.jip.2014.11.007 [doi]
PST - ppublish
SO  - J Invertebr Pathol. 2015 Jan;124:98-106. doi: 10.1016/j.jip.2014.11.007. Epub 
      2014 Dec 5.