PMID- 25487973
OWN - NLM
STAT- MEDLINE
DCOM- 20160325
LR  - 20181113
IS  - 1573-0832 (Electronic)
IS  - 0301-486X (Linking)
VI  - 179
IP  - 1-2
DP  - 2015 Feb
TI  - Immunization with recombinant Pb27 protein reduces the levels of pulmonary 
      fibrosis caused by the inflammatory response against Paracoccidioides 
      brasiliensis.
PG  - 31-43
LID - 10.1007/s11046-014-9815-8 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic mycosis in which the host response to 
      the infectious agent typically consists of a chronic granulomatous inflammatory 
      process. This condition causes lesions that impair lung function and lead to 
      chronic pulmonary insufficiency resulting from fibrosis development, which is a 
      sequel and disabling feature of the disease. The rPb27 protein has been studied 
      for prophylactic and therapeutic treatment against PCM. Previous studies from our 
      laboratory have shown a protective effect of rPb27 against PCM. However, these 
      studies have not determined whether rPb27 immunization prevents lung fibrosis. We 
      therefore conducted this study to investigate fibrosis resulting from infection 
      by Paracoccidioides brasiliensis in the lungs of animals immunized with rPb27. 
      Animals were immunized with rPb27 and subsequently infected with a virulent 
      strain of P. brasiliensis. Fungal load was evaluated by counting colony-forming 
      units, and Masson's trichrome staining was performed to evaluate fibrosis at 30 
      and 90 days post-infection. The levels of CCR7, active caspase 3, collagen and 
      cytokines were analyzed. At the two time intervals mentioned, the rPb27 group 
      showed lower levels of fibrosis on histology and reduced levels of collagen and 
      the chemokine receptor CCR7 in the lungs. CCR7 was detected at higher levels in 
      the control groups that developed very high levels of pulmonary fibrosis. 
      Additionally, the immunized groups showed high levels of active caspase 3, IFN-gamma, 
      TGF-beta and IL-10 in the early phase of P. brasiliensis infection. Immunization 
      with Pb27, in addition to its protective effect, was shown to prevent pulmonary 
      fibrosis.
FAU - Morais, Elis Araujo
AU  - Morais EA
AD  - Department of Biochemistry and Immunology, Institute of Biological Sciences, 
      Federal University of Minas Gerais, Belo Horizonte, Brazil.
FAU - Martins, Estefania Mara do Nascimento
AU  - Martins EM
FAU - Boelone, Jankerle Neves
AU  - Boelone JN
FAU - Gomes, Dawidson Assis
AU  - Gomes DA
FAU - Goes, Alfredo Miranda
AU  - Goes AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141209
PL  - Netherlands
TA  - Mycopathologia
JT  - Mycopathologia
JID - 7505689
RN  - 0 (Antifungal Agents)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Ccr7 protein, mouse)
RN  - 0 (Fungal Proteins)
RN  - 0 (Fungal Vaccines)
RN  - 0 (IL10 protein, mouse)
RN  - 0 (Pb27 protein, Paracoccidioides brasiliensis)
RN  - 0 (Receptors, CCR7)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 8VZV102JFY (Fluconazole)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Antifungal Agents/therapeutic use
MH  - Antigens, Fungal/immunology
MH  - Caspase 3/metabolism
MH  - Collagen/metabolism
MH  - Fluconazole/therapeutic use
MH  - Fungal Proteins/administration & dosage/*immunology
MH  - Fungal Vaccines/immunology
MH  - Immunization
MH  - Inflammation/immunology/prevention & control
MH  - Interferon-gamma/metabolism
MH  - Interleukin-10/metabolism
MH  - Lung/microbiology/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*immunology/microbiology/prevention & control
MH  - Propionibacterium acnes/immunology
MH  - Pulmonary Fibrosis/immunology/microbiology/*prevention & control
MH  - Receptors, CCR7/metabolism
MH  - Recombinant Proteins/administration & dosage/immunology
MH  - Transforming Growth Factor beta/metabolism
EDAT- 2014/12/10 06:00
MHDA- 2016/03/26 06:00
CRDT- 2014/12/10 06:00
PHST- 2014/06/10 00:00 [received]
PHST- 2014/09/17 00:00 [accepted]
PHST- 2014/12/10 06:00 [entrez]
PHST- 2014/12/10 06:00 [pubmed]
PHST- 2016/03/26 06:00 [medline]
AID - 10.1007/s11046-014-9815-8 [doi]
PST - ppublish
SO  - Mycopathologia. 2015 Feb;179(1-2):31-43. doi: 10.1007/s11046-014-9815-8. Epub 
      2014 Dec 9.