PMID- 25488445
OWN - NLM
STAT- MEDLINE
DCOM- 20150821
LR  - 20181113
IS  - 0971-5916 (Print)
IS  - 0975-9174 (Electronic)
IS  - 0971-5916 (Linking)
VI  - 140
IP  - 4
DP  - 2014 Oct
TI  - Double positive CD4+CD8+ T cells: key suppressive role in the production of 
      autoantibodies in systemic lupus erythematosus.
PG  - 513-9
AB  - BACKGROUND & OBJECTIVES: The presence of CD4+CD8+ (double positive) T cells (DPT) 
      in the target organs of several autoimmune diseases has been reported. The aim of 
      this study was to investigate the pathogenic role of DPT in systemic lupus 
      erythematosus (SLE). METHODS: A total of 175 SLE cases and 125 matched healthy 
      controls were investigated for CD3+, CD4+, CD8+ lymphocytes and DPT by flow 
      cytometry. Serum samples from SLE patients and controls were tested for 
      antinuclear antibody (ANA), anti-double strain deoxyribonucleic acid 
      (anti-dsDNA), anti-U1 ribonucleoprotein (anti-U1 RNP), anti-sjogren syndrome A 
      (anti-SSA), anti-ribosomal P protein (anti-rib-P), anti-Smith (anti-Sm), 
      anti-Sjogren syndrome B (anti-SSB), complement 3 (C3) and complement 4 (C4). 
      RESULTS: The DPT median and 5-95 per cent range of SLE cases and healthy controls 
      were 0.50 [0.10-2.60] and 0.80 [0.20-2.74] respectively (P<0.001). SLE patients 
      were divided into a >/=1:1000 subgroup and a <1:1000 subgroup according to the ANA 
      titre. The DPT of the former subgroup was significantly lower than that of the 
      latter (P=0.032). The DPT medians of positive subgroups with anti-dsDNA 
      (P<0.001), anti-U1RNP (P=0.018), anti-SSA (P=0.021) or anti-rib-P (P=0.039) were 
      also significantly lower than the negative subgroups. Likewise, DPT was 
      significantly lower in SLE subgroups with low concentration of C3 or C4 than 
      those with high concentration (P<0.006). INTERPRETATION & CONCLUSIONS: Our 
      findings show that the DPT cells may play a key suppressive role in the 
      production of autoantibodies in SLE. Direct evidence that DPT regulates the 
      pathogenesis of SLE needs to be investigated in future work.
FAU - Wu, Yongkang
AU  - Wu Y
FAU - Cai, Bei
AU  - Cai B
FAU - Feng, Weihua
AU  - Feng W
FAU - Yang, Bin
AU  - Yang B
FAU - Huang, Zhuochun
AU  - Huang Z
FAU - Zuo, Chuan
AU  - Zuo C
FAU - Wang, Lanlan
AU  - Wang L
AD  - Department of Laboratory Medicine, West China Hospital affiliated Sichuan 
      University, Chengdu, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Indian J Med Res
JT  - The Indian journal of medical research
JID - 0374701
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Autoantibodies)
SB  - IM
MH  - Adult
MH  - Antibodies, Antinuclear/blood/immunology/isolation & purification
MH  - Autoantibodies/blood/*immunology/isolation & purification
MH  - CD4-Positive T-Lymphocytes/*immunology/pathology
MH  - CD8-Positive T-Lymphocytes/*immunology/pathology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Lupus Erythematosus, Systemic/blood/*immunology
MH  - Male
MH  - Middle Aged
PMC - PMC4277137
EDAT- 2014/12/10 06:00
MHDA- 2015/08/22 06:00
PMCR- 2014/10/01
CRDT- 2014/12/10 06:00
PHST- 2014/12/10 06:00 [entrez]
PHST- 2014/12/10 06:00 [pubmed]
PHST- 2015/08/22 06:00 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - IndianJMedRes_2014_140_4_513_146239 [pii]
AID - IJMR-140-513 [pii]
PST - ppublish
SO  - Indian J Med Res. 2014 Oct;140(4):513-9.