PMID- 25490390
OWN - NLM
STAT- MEDLINE
DCOM- 20150310
LR  - 20141224
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 456
IP  - 1
DP  - 2015 Jan 2
TI  - Exosome from chaperone-rich cell lysates-loaded dendritic cells produced by 
      CELLine 1000 culture system exhibits potent immune activity.
PG  - 513-8
LID - S0006-291X(14)02159-7 [pii]
LID - 10.1016/j.bbrc.2014.11.117 [doi]
AB  - Dendritic cells (DCs) pulsed with exosomes can stimulate efficient cytotoxic 
      T-lymphocyte responses and anti-tumor immunity. However, the quantity of 
      DC-derived exosomes (DCex) obtained from various culture systems is very low, 
      which is a significant practical issue hampering progress in this research area 
      and needs to be addressed. Gliomas were particularly aggressive, with high 
      morbidity and mortality, indicating that this is a form of incurable highly 
      malignant tumor of the brain with poor prognosis. In the present study, we 
      demonstrate that the CELLine 1000 culture system can dramatically increase the 
      production of DCex. The morphology, phenotype and immune molecules of these DCex 
      were found to be identical to those using traditional methods. Our researches 
      supply a cost-effective, useful method for significantly increasing the quantity 
      of exosomes. In addition, GL261 glioma cells were chosen to separate 
      chaperone-rich cell lysates (CRCL). The results indicate that CRCL-GL261 cell 
      lysates can trigger the most intense expression of immune molecules on DCex or 
      DCs, which has important implications for the research into tumor treatment and 
      diagnosis.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Bu, Ning
AU  - Bu N
AD  - Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China. Electronic address: ningbuxa@163.com.
FAU - Wu, Haiqin
AU  - Wu H
AD  - Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China.
FAU - Zhang, Guilian
AU  - Zhang G
AD  - Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China.
FAU - Ma, Xiaoling
AU  - Ma X
AD  - Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China.
FAU - Zhao, Ping
AU  - Zhao P
AD  - Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China.
FAU - Zhai, Nina
AU  - Zhai N
AD  - Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China.
FAU - Xiang, Li
AU  - Xiang L
AD  - Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China.
FAU - Cao, Huifang
AU  - Cao H
AD  - Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China.
FAU - Yang, Xinli
AU  - Yang X
AD  - Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China.
FAU - Liu, Jingjie
AU  - Liu J
AD  - Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141206
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Culture Media)
RN  - 0 (Molecular Chaperones)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/chemistry
MH  - Brain Neoplasms/*immunology/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Culture Media
MH  - Dendritic Cells/metabolism
MH  - Exosomes/*metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Glioma/*immunology/pathology
MH  - Lymphocyte Culture Test, Mixed
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Immunoelectron
MH  - Molecular Chaperones/*immunology
MH  - Phenotype
MH  - Prognosis
MH  - T-Lymphocytes
MH  - T-Lymphocytes, Cytotoxic/cytology
OTO - NOTNLM
OT  - Chaperone-rich cell lysates (CRCL)
OT  - DC-derived exosomes (DCex)
OT  - Dendritic cells (DCs)
OT  - Glioma
EDAT- 2014/12/10 06:00
MHDA- 2015/03/11 06:00
CRDT- 2014/12/10 06:00
PHST- 2014/11/20 00:00 [received]
PHST- 2014/11/28 00:00 [accepted]
PHST- 2014/12/10 06:00 [entrez]
PHST- 2014/12/10 06:00 [pubmed]
PHST- 2015/03/11 06:00 [medline]
AID - S0006-291X(14)02159-7 [pii]
AID - 10.1016/j.bbrc.2014.11.117 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Jan 2;456(1):513-8. doi: 
      10.1016/j.bbrc.2014.11.117. Epub 2014 Dec 6.