PMID- 25491616
OWN - NLM
STAT- MEDLINE
DCOM- 20150824
LR  - 20221207
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 18
IP  - 22
DP  - 2014 Nov
TI  - Experimental study on transplantation of autologous minced muscle with human 
      umbilical cord mesenchymal stem cells for urethral reconstruction.
PG  - 3412-9
LID - 8080 [pii]
AB  - OBJECTIVE: To investigate the feasibility and efficiency of the autologous minced 
      muscle-derived regenerate as a living patch graft for the urethral reconstruction 
      with the assistance of human umbilical cord mesenchymal stem cells (hUC-MSCs) in 
      the rabbit model. MATERIALS AND METHODS: The hUC-MSCs and minced-muscle-derived 
      regenerate tissue was prepared and used as potential autologous patch graft for 
      the reconstruction of the defective rabbit urethra. Animal with autologous free 
      skeletal muscle piece was observed as control. Histopathological and 
      immunohistochemical staining methods were adopted to evaluate the regenerating 
      effect; the reconstructive effects in New Zealand rabbit were observed in both 
      groups by retrograde urethrography and urinary microscopy. RESULTS: The 
      expression of desmin and anti-human specific nuclear antigen (ANA) were positive 
      for co-culture microsomes in vivo and in vitro; after the reconstructive surgery, 
      histopathological studies revealed fibrous connective tissues and abundant muscle 
      fibers constituted the main body of the patch-grafted urethra. Urethrography and 
      urethroscopy showed no urethral obstruction, stenosis, fistula or diverticula 
      anomaly existed in experimental group. CONCLUSIONS: Our preclinical study showed 
      that the hUC-MSCs and minced-muscle-derived regenerate could be used as an 
      autologous pre-vascularized living patch graft for urethral reconstruction.
FAU - Xu, Y
AU  - Xu Y
AD  - Urology Department of the PLA General Hospital, Beijing, China. 
      yongyang826@yahoo.cn.
FAU - Sun, D-C
AU  - Sun DC
FAU - Wei, Z-T
AU  - Wei ZT
FAU - Hong, B-F
AU  - Hong BF
FAU - Yang, Y
AU  - Yang Y
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
SB  - IM
MH  - Animals
MH  - Coculture Techniques
MH  - Female
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Muscle, Skeletal/cytology/*transplantation
MH  - Rabbits
MH  - Plastic Surgery Procedures/*methods
MH  - Transplantation, Autologous/methods
MH  - Umbilical Cord/cytology/*transplantation
MH  - Urethra/pathology/physiology/*surgery
EDAT- 2014/12/11 06:00
MHDA- 2015/08/25 06:00
CRDT- 2014/12/11 06:00
PHST- 2014/12/11 06:00 [entrez]
PHST- 2014/12/11 06:00 [pubmed]
PHST- 2015/08/25 06:00 [medline]
AID - 8080 [pii]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2014 Nov;18(22):3412-9.