PMID- 25491813
OWN - NLM
STAT- MEDLINE
DCOM- 20150821
LR  - 20141210
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 45
DP  - 2014 Dec
TI  - Synthesis of new antibacterial composite coating for titanium based on highly 
      ordered nanoporous silica and silver nanoparticles.
PG  - 146-53
LID - S0928-4931(14)00552-9 [pii]
LID - 10.1016/j.msec.2014.08.057 [doi]
AB  - Infection is the most common factor that leads to dental titanium implant 
      failure. Antibacterial implant surfaces based on nano-scale modifications of the 
      titanium appear as an attractive strategy for control of peri-implantitis. In the 
      present work, the preparation and antibacterial properties of a novel composite 
      coating for titanium based on nanoporous silica and silver nanoparticles are 
      presented. Starch-capped silver nanoparticles (AgNPs) were synthesized and then 
      incorporated into sol-gel based solution system. The AgNP-doped nanoporous silica 
      coatings were prepared on titanium surface using a combined sol-gel and 
      evaporation-induced self-assembly (EISA) method. The coating nanostructure was 
      characterized by XRD, SEM-EDX, and HR-TEM. Antibacterial activity was evaluated 
      against Aggregatibacter actinomycetemcomitans, a representative pathogen of 
      dental peri-implantitis. Colony-forming units (CFUs) were counted within the 
      biofilm and at the planktonic state. Biofilm development was quantified using 
      crystal violet staining and viability of adherent bacteria was confirmed with the 
      Live/Dead fluorescence assay. Silica-based composite coating containing AgNPs 
      (AgNP/NSC) was prepared on titanium surface by direct incorporation of AgNP 
      suspension into the sol-gel system. The self-assembly technique enabled the 
      spontaneous formation of a highly ordered nanoporosity in the coating structure, 
      which is a desired property for osseointegration aspects of titanium implant 
      surface. AgNP/NSC coating produces a strong antibacterial effect on titanium 
      surface by not only killing the adherent bacteria but also reducing the extent of 
      biofilm formation. Biofilm survival is reduced by more than 70% on the 
      AgNP/NSC-modified titanium surface, compared to the control. This antibacterial 
      effect was verified for up to 7 days of incubation. The long-term antibacterial 
      activity exhibited by the nanostructured AgNP/NSC-titanium surface against A. 
      actinomycetemcomitans suggests that this type of nano-scale surface modification 
      is a promissory strategy to control infections associated with dental implant 
      rehabilitation.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Massa, Miguel A
AU  - Massa MA
AD  - Laboratory of Nanobiomaterials, Institute for Research in Dental Sciences, 
      Faculty of Dentistry, University of Chile, Independencia, Santiago, Chile.
FAU - Covarrubias, Cristian
AU  - Covarrubias C
AD  - Laboratory of Nanobiomaterials, Institute for Research in Dental Sciences, 
      Faculty of Dentistry, University of Chile, Independencia, Santiago, Chile. 
      Electronic address: ccovarru@u.uchile.cl.
FAU - Bittner, Mauricio
AU  - Bittner M
AD  - Laboratory of Oral Microbiology and Biotechnology, Faculty of Biological 
      Sciences, Andres Bello University, Santiago, Chile; Laboratory of Oral 
      Microbiology and Biotechnology, Faculty of Dentistry, Andres Bello University, 
      Santiago, Chile.
FAU - Fuentevilla, Ignacio Andres
AU  - Fuentevilla IA
AD  - Laboratory of Oral Microbiology and Biotechnology, Faculty of Biological 
      Sciences, Andres Bello University, Santiago, Chile.
FAU - Capetillo, Pavel
AU  - Capetillo P
AD  - Laboratory of Nanobiomaterials, Institute for Research in Dental Sciences, 
      Faculty of Dentistry, University of Chile, Independencia, Santiago, Chile.
FAU - Von Marttens, Alfredo
AU  - Von Marttens A
AD  - Laboratory of Nanobiomaterials, Institute for Research in Dental Sciences, 
      Faculty of Dentistry, University of Chile, Independencia, Santiago, Chile.
FAU - Carvajal, Juan Carlos
AU  - Carvajal JC
AD  - Laboratory of Nanobiomaterials, Institute for Research in Dental Sciences, 
      Faculty of Dentistry, University of Chile, Independencia, Santiago, Chile.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140904
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Dental Implants)
RN  - 0 (Gels)
RN  - 3M4G523W1G (Silver)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Aggregatibacter actinomycetemcomitans/drug effects/physiology
MH  - Anti-Bacterial Agents/*chemical synthesis/chemistry/pharmacology
MH  - Biofilms/drug effects/growth & development
MH  - Coated Materials, Biocompatible/*chemical synthesis/chemistry/pharmacology
MH  - Dental Implants
MH  - Gels/chemistry
MH  - Metal Nanoparticles/*chemistry
MH  - Nanopores
MH  - Silicon Dioxide/*chemistry
MH  - Silver/*chemistry/metabolism
MH  - Surface Properties
MH  - Titanium/*chemistry
MH  - X-Ray Diffraction
OTO - NOTNLM
OT  - Antibacterial surface
OT  - Peri-implantitis
OT  - Silver nanoparticles
OT  - Titanium
EDAT- 2014/12/11 06:00
MHDA- 2015/08/22 06:00
CRDT- 2014/12/11 06:00
PHST- 2014/05/12 00:00 [received]
PHST- 2014/08/07 00:00 [revised]
PHST- 2014/08/29 00:00 [accepted]
PHST- 2014/12/11 06:00 [entrez]
PHST- 2014/12/11 06:00 [pubmed]
PHST- 2015/08/22 06:00 [medline]
AID - S0928-4931(14)00552-9 [pii]
AID - 10.1016/j.msec.2014.08.057 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2014 Dec;45:146-53. doi: 
      10.1016/j.msec.2014.08.057. Epub 2014 Sep 4.