PMID- 25492210
OWN - NLM
STAT- MEDLINE
DCOM- 20150807
LR  - 20141210
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 47
DP  - 2015 Feb
TI  - Porous titania surfaces on titanium with hierarchical macro- and mesoporosities 
      for enhancing cell adhesion, proliferation and mineralization.
PG  - 376-83
LID - S0928-4931(14)00722-X [pii]
LID - 10.1016/j.msec.2014.11.021 [doi]
AB  - Titanium received a macroporous titania surface layer by anodization, which 
      contains open pores with average pore diameter around 5 mum. An additional 
      mesoporous titania top layer following the contour of the macropores, of 100-200 
      nm thickness and with a pore diameter of 10nm, was formed by using the 
      evaporation-induced self-assembly (EISA) method with titanium (IV) tetraethoxide 
      as the precursor. A coherent laminar titania surface layer was thus obtained, 
      creating a hierarchical macro- and mesoporous surface that was characterized by 
      high-resolution electron microscopy. The interfacial bonding between the surface 
      layers and the titanium matrix was characterized by the scratch test that 
      confirmed a stable and strong bonding of titania surface layers on titanium. The 
      wettability to water and the effects on the osteosarcoma cell line (SaOS-2) 
      proliferation and mineralization of the formed titania surface layers were 
      studied systematically by cell culture and scanning electron microscopy. The 
      results proved that the porous titania surface with hierarchical macro- and 
      mesoporosities was hydrophilic that significantly promoted cell attachment and 
      spreading. A synergistic role of the hierarchical macro- and mesoporosities was 
      revealed in terms of enhancing cell adhesion, proliferation and mineralization, 
      compared with the titania surface with solo scale topography.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Han, Guang
AU  - Han G
AD  - Department of Materials and Environmental Chemistry, Arrhenius Laboratory, 
      Stockholm University, 10691 Stockholm, Sweden.
FAU - Muller, Werner E G
AU  - Muller WE
AD  - ERC Advanced Grant Research Group at the Institute for Physiological Chemistry, 
      University Medical Center of the Johannes Gutenberg University Mainz, Duesbergweg 
      6, D-55128 Mainz, Germany.
FAU - Wang, Xiaohong
AU  - Wang X
AD  - ERC Advanced Grant Research Group at the Institute for Physiological Chemistry, 
      University Medical Center of the Johannes Gutenberg University Mainz, Duesbergweg 
      6, D-55128 Mainz, Germany.
FAU - Lilja, Louise
AU  - Lilja L
AD  - Department of Materials and Environmental Chemistry, Arrhenius Laboratory, 
      Stockholm University, 10691 Stockholm, Sweden; Department of Physics, Chemistry 
      and Biology, Linkoping University, SE-581 83 Linkoping, Sweden.
FAU - Shen, Zhijian
AU  - Shen Z
AD  - Department of Materials and Environmental Chemistry, Arrhenius Laboratory, 
      Stockholm University, 10691 Stockholm, Sweden. Electronic address: 
      shen@mmk.su.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141108
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 15FIX9V2JP (titanium dioxide)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Bone Neoplasms/drug therapy
MH  - Calcification, Physiologic/*drug effects
MH  - Cell Adhesion/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Humans
MH  - Microscopy, Electron, Scanning/methods
MH  - Osteosarcoma/drug therapy
MH  - Porosity
MH  - Surface Properties
MH  - Titanium/*pharmacology
OTO - NOTNLM
OT  - Cell adhesion
OT  - Cell proliferation
OT  - Porosity
OT  - Surface topography
OT  - Titanium oxide
EDAT- 2014/12/11 06:00
MHDA- 2015/08/08 06:00
CRDT- 2014/12/11 06:00
PHST- 2014/06/17 00:00 [received]
PHST- 2014/10/03 00:00 [revised]
PHST- 2014/11/06 00:00 [accepted]
PHST- 2014/12/11 06:00 [entrez]
PHST- 2014/12/11 06:00 [pubmed]
PHST- 2015/08/08 06:00 [medline]
AID - S0928-4931(14)00722-X [pii]
AID - 10.1016/j.msec.2014.11.021 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2015 Feb;47:376-83. doi: 
      10.1016/j.msec.2014.11.021. Epub 2014 Nov 8.