PMID- 25492485
OWN - NLM
STAT- MEDLINE
DCOM- 20150709
LR  - 20181113
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 36
IP  - 4
DP  - 2015 Apr
TI  - A polysaccharide from mushroom Huaier retards human hepatocellular carcinoma 
      growth, angiogenesis, and metastasis in nude mice.
PG  - 2929-36
LID - 10.1007/s13277-014-2923-8 [doi]
AB  - Mushroom Huaier has become a focus of interest in the treatment of hepatocellular 
      carcinoma (HCC). Presently, we isolated and purified one polysaccharide from this 
      mushroom. This study aimed to investigate the effects of SP1 on tumor growth and 
      metastasis in a HCC xenograft model and explore its possible mechanism of action. 
      Our results showed that SP1 not only significantly inhibited the proliferation of 
      SMMC-7721 cells in vitro at the concentration ranging from 0 to 800 mug/ml but 
      also suppressed the HCC tumor growth and metastatic nodules to the lung in 
      SMMC-7721-bearing mice by oral administration at three doses of 30, 60, and 
      120 mg/kg. Concomitantly, immunohistochemistry analysis of tumor tissues 
      identified that SP1 administration at three doses significantly inhibited the in 
      vivo cancer cell proliferation and microvessel density (MVD) formation, evidenced 
      by a low proliferating cell nuclear antigen (PCNA) and CD34 expression, but 
      increased the percentage of terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL)-positive cells. Keeping in line with this observation, 
      SP1 treatment decreased serum matrix metalloproteinase (MMP) 2 and vascular 
      endothelial growth factor (VEGF) levels, downregulated the protein expression of 
      hypoxia-inducible factor (HIF)-1alpha, VEGF, MMP2, bcl-2, N-cadherin, signal 
      transducer and activator of transcription 3 (STAT3), and metadherin (MTDH), and 
      upregulated bax and NE-cadherin protein expression in tumor tissues. Taken 
      together, our data suggest that SP1 appears to be a promising chemopreventive 
      agent for the tumorigenesis and metastasis in patients with HCC, especially at 
      advanced stages.
FAU - Zou, Yanmei
AU  - Zou Y
AD  - Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Xiong, Hua
AU  - Xiong H
FAU - Xiong, Huihua
AU  - Xiong H
FAU - Lu, Tao
AU  - Lu T
FAU - Zhu, Feng
AU  - Zhu F
FAU - Luo, Zhiyong
AU  - Luo Z
FAU - Yuan, Xianglin
AU  - Yuan X
FAU - Wang, Yihua
AU  - Wang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141211
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Agaricales/chemistry
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Carcinogenesis/*drug effects
MH  - Carcinoma, Hepatocellular/*drug therapy/genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Liver Neoplasms/*drug therapy/genetics/pathology
MH  - Mice
MH  - Neoplasm Metastasis
MH  - Neoplasm Proteins/biosynthesis
MH  - Polysaccharides/*administration & dosage/chemistry
MH  - Xenograft Model Antitumor Assays
EDAT- 2014/12/11 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/12/11 06:00
PHST- 2014/09/02 00:00 [received]
PHST- 2014/11/28 00:00 [accepted]
PHST- 2014/12/11 06:00 [entrez]
PHST- 2014/12/11 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.1007/s13277-014-2923-8 [doi]
PST - ppublish
SO  - Tumour Biol. 2015 Apr;36(4):2929-36. doi: 10.1007/s13277-014-2923-8. Epub 2014 
      Dec 11.