PMID- 25493168
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151026
LR  - 20200929
IS  - 2008-076X (Print)
IS  - 2008-0778 (Electronic)
IS  - 2008-0778 (Linking)
VI  - 6
IP  - 2
DP  - 2012 Jul
TI  - Nitric oxide-induced polycystic ovaries in the wistar rat.
PG  - 111-6
AB  - BACKGROUND: Nitric oxide (NO) involves in polycystic ovary syndrome (PCOS), a 
      cause of infertility in women during the reproductive age. The PCOS is now 
      categorized as an inflammatory phenomenon. The aim of this study was to evaluate 
      the role of NO, a proinflammatory agent, in this syndrome at histological and 
      biochemical levels. MATERIALS AND METHODS: In this experimental study, animals 
      were female Wistar rats (weighing 200-250 g) kept under standard conditions. 
      L-Arginine (50-200 mg/kg), a precursor of NO, was injected intra-peritoneally 
      (i.p.) through a period ranging from 9 to14 days/ once a day. The rats' estrous 
      cycle was studied using Papanicolaou test; those showing phase of Diestrous were 
      grouped into experimental and control groups. The control group solely received 
      saline (1 ml/kg, i.p.) throughout all experiments. To evaluate the inflammatory 
      effect of NO, the rats were treated an anti-inflammatory agent, naloxone 
      hydrochloride (0.4 mg/kg, i.p.), prior to L-arginine. At the end of the treatment 
      period all animals' ovaries were assessed for histopathological and histochemical 
      investigations. Also, activation of NO synthase (NOS) in the experiments was 
      studied using NADPH-diaphorase technique. RESULTS: The ovaries of rats treated 
      with L-arginine showed polycystic characteristics in contrast to those collected 
      from control or naloxone pretreated groups, based on image analysis. A difference 
      in enzyme activation was also shown in the sections that belonged to the groups 
      that received L-arginine when compared with the pre-naloxone and control groups. 
      CONCLUSION: Based on these results, we believe that NO may play a major role in 
      the pathophysiology of PCOS.
FAU - Hassani, Fatemeh
AU  - Hassani F
AD  - Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, 
      Iran.
FAU - Karami, Manizheh
AU  - Karami M
AD  - Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, 
      Iran.
FAU - 1, Ph D 1
AU  - 1 PD
AD  - Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, 
      Iran.
FAU - Jalali Nadoushan, Mohammad Reza
AU  - Jalali Nadoushan MR
AD  - Department of Pathology, School of Medicine, Shahed University, Tehran, Iran.
FAU - Yazdi, Poopak Eftekhari
AU  - Yazdi PE
AD  - Department of Embryology, Reproductive Biomedicine Research Center, Royan 
      Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20120619
PL  - Iran
TA  - Int J Fertil Steril
JT  - International journal of fertility & sterility
JID - 101487941
PMC - PMC4258239
OTO - NOTNLM
OT  - L-Arginine
OT  - NADPH-Diaphorase
OT  - Naloxone
OT  - Nitric oxide
OT  - PCOS
EDAT- 2012/07/01 00:00
MHDA- 2012/07/01 00:01
PMCR- 2012/07/01
CRDT- 2014/12/11 06:00
PHST- 2011/06/14 00:00 [received]
PHST- 2012/02/05 00:00 [accepted]
PHST- 2014/12/11 06:00 [entrez]
PHST- 2012/07/01 00:00 [pubmed]
PHST- 2012/07/01 00:01 [medline]
PHST- 2012/07/01 00:00 [pmc-release]
PST - ppublish
SO  - Int J Fertil Steril. 2012 Jul;6(2):111-6. Epub 2012 Jun 19.