PMID- 25493640
OWN - NLM
STAT- MEDLINE
DCOM- 20150916
LR  - 20181113
IS  - 1949-1042 (Electronic)
IS  - 1949-1034 (Print)
IS  - 1949-1034 (Linking)
VI  - 5
IP  - 6
DP  - 2014
TI  - Functional redundancy in the nuclear compartmentalization of the late-replicating 
      genome.
PG  - 626-35
LID - 10.4161/19491034.2014.990863 [doi]
AB  - The eukaryotic nucleus is structurally and functionally organized, as reflected 
      in the distribution of its protein and DNA components. The genome itself is 
      segregated into euchromatin and heterochromatin that replicate in a distinct 
      spatio-temporal manner. We used a combination of fluorescence in situ 
      hybridization (FISH) and DamID to investigate the localization of the early and 
      late replicating components of the genome in a lymphoblastoid cell background. 
      Our analyses revealed that the bulk of late replicating chromatin localizes to 
      the nuclear peripheral heterochromatin (PH) in a chromosome size and gene density 
      dependent manner. Late replicating DNA on small chromosomes exhibits a much lower 
      tendency to localize to PH and tends to associate with alternate repressive 
      subcompartments such as pericentromeric (PCH) and perinucleolar heterochromatin 
      (PNH). Furthermore, multicolor FISH analysis revealed that late replicating loci, 
      particularly on the smaller chromosomes, may associate with any of these 3 
      repressive subcompartments, including more than one at the same time. These 
      results suggest a functional equivalence or redundancy among the 3 
      subcompartments. Consistent with this notion, disruption of nucleoli resulted in 
      an increased association of late replicating loci with peripheral 
      heterochromatin. Our analysis reveals that rather than considering the 
      morphologically distinct PH, PCH and PNH as individual subcompartments, they 
      should be considered in aggregate as a functional compartment for late 
      replicating chromatin.
FAU - Ragoczy, Tobias
AU  - Ragoczy T
AD  - a Division of Basic Sciences ; Fred Hutchinson Cancer Research Center ; Seattle , 
      WA USA.
FAU - Telling, Agnes
AU  - Telling A
FAU - Scalzo, David
AU  - Scalzo D
FAU - Kooperberg, Charles
AU  - Kooperberg C
FAU - Groudine, Mark
AU  - Groudine M
LA  - eng
GR  - R01 HL065440/HL/NHLBI NIH HHS/United States
GR  - R37 DK044746/DK/NIDDK NIH HHS/United States
GR  - DK44746/DK/NIDDK NIH HHS/United States
GR  - HL065440/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Nucleus
JT  - Nucleus (Austin, Tex.)
JID - 101518322
RN  - 0 (Euchromatin)
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Cell Compartmentation/*genetics
MH  - Cell Line
MH  - Cell Nucleus/*genetics/ultrastructure
MH  - Chromosomes/genetics
MH  - DNA Replication/*genetics
MH  - Euchromatin/genetics
MH  - Genome, Human
MH  - Heterochromatin/*genetics/ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
PMC - PMC4615584
OTO - NOTNLM
OT  - Chr, chromosome
OT  - DamID
OT  - DamID, Dam identification
OT  - EU, 5-Ethynyl uridine
OT  - FISH, fluorescence in situ hybridization
OT  - LAD, lamina associated domain
OT  - NOR, nucleolar organizing region
OT  - PCH, pericentromeric heterochromatin
OT  - PH, peripheral heterochromatin
OT  - PNH, perinucleolar heterochromatin
OT  - heterochromatin
OT  - localization
OT  - nuclear organization
OT  - nuclear periphery, pericentromeric heterochromatin
OT  - perinucleolar heterochromatin
OT  - replication timing
OT  - repressive compartments
EDAT- 2014/12/11 06:00
MHDA- 2015/09/17 06:00
PMCR- 2015/12/10
CRDT- 2014/12/11 06:00
PHST- 2014/12/11 06:00 [entrez]
PHST- 2014/12/11 06:00 [pubmed]
PHST- 2015/09/17 06:00 [medline]
PHST- 2015/12/10 00:00 [pmc-release]
AID - 990863 [pii]
AID - 10.4161/19491034.2014.990863 [doi]
PST - ppublish
SO  - Nucleus. 2014;5(6):626-35. doi: 10.4161/19491034.2014.990863.