PMID- 25494574
OWN - NLM
STAT- MEDLINE
DCOM- 20151021
LR  - 20161125
IS  - 1365-2826 (Electronic)
IS  - 0953-8194 (Linking)
VI  - 27
IP  - 2
DP  - 2015 Feb
TI  - Carbon monoxide and nitric oxide interactions in magnocellular neurosecretory 
      neurones during water deprivation.
PG  - 111-22
LID - 10.1111/jne.12245 [doi]
AB  - Nitric oxide (NO) and carbon monoxide (CO) are diffusible gas messengers in the 
      brain. Previously, we have shown their independent involvement in central 
      fluid/electrolyte homeostasis control. In the present study, we investigated a 
      possible functional interaction between NO/CO in the regulation of vasopressin 
      (VP) and oxytocin (OT) magnocellular neurosecretory cells (MNCs) activity in 
      euhydrated (EU) and dehydrated [48-h water-deprived (48WD)] rats. Using brain 
      slices from EU and 48WD rats, we measured, by immunohistochemistry, the 
      expression of neuronal NO synthase (nNOS, which synthesises NO) and 
      haeme-oxygenase (HO-1, which synthesises CO) in the hypothalamic supraoptic 
      nucleus (SON). In addition, we used patch-clamp electrophysiology to investigate 
      whether regulation of SON MNC firing activity by endogenous CO was dependent on 
      NO bioavailability and GABAergic inhibitory synaptic function. We found a 
      proportion of OT and VP SON MNCs in EU rats to co-express both of HO-1 and nNOS 
      (33.2 +/- 2.9% and 15.3 +/- 1.4%, respectively), which was increased in 48WD rats 
      (55.5 +/- 0.9% and 21.0 +/- 1.7%, respectively, P < 0.05 for both). Inhibition of 
      endogenous HO activity [chromium mesoporphyrin IX chloride (CrMP) 20 mum] induced 
      MNC membrane hyperpolarisation and decreased firing activity, and these effects 
      were blunted by previous blockade of endogenous NOS activity (l-NAME, 2 mm) or 
      blockade of inhibitory GABA function [Picrotoxin (Sigma-Aldrich, St Louis, MO, 
      USA), 50 mum]. No significant changes in SON NO bioavailability (4,5 
      diaminofluorescein diacetate fluorescence) were observed after CrMP treatment. 
      Taken together, our results support a state-dependent functional 
      inter-relationship between NO and CO in MNCs, in which CO acts as an excitatory 
      gas molecule, whose effects are largely dependent on interactions with the 
      inhibitory SON signals NO and GABA.
CI  - (c) 2014 British Society for Neuroendocrinology.
FAU - Reis, W L
AU  - Reis WL
AD  - Department of Physiology, Georgia Regents University, Augusta, GA, USA; 
      Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao 
      Paulo, Ribeirao Preto, SP, Brazil.
FAU - Biancardi, V C
AU  - Biancardi VC
FAU - Son, S
AU  - Son S
FAU - Antunes-Rodrigues, J
AU  - Antunes-Rodrigues J
FAU - Stern, J E
AU  - Stern JE
LA  - eng
GR  - HL113801/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 11000-17-2 (Vasopressins)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 50-56-6 (Oxytocin)
RN  - 7U1EE4V452 (Carbon Monoxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
SB  - IM
MH  - Animals
MH  - Carbon Monoxide/*metabolism
MH  - Heme Oxygenase (Decyclizing)/metabolism
MH  - Male
MH  - Neurons/*metabolism
MH  - Nitric Oxide/*metabolism
MH  - Nitric Oxide Synthase Type I/metabolism
MH  - Oxytocin/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Supraoptic Nucleus/cytology/*metabolism
MH  - Vasopressins/metabolism
MH  - Water Deprivation/*physiology
OTO - NOTNLM
OT  - carbon monoxide
OT  - nitric oxide
OT  - osmotic stimulation
OT  - oxytocin
OT  - vasopressin
EDAT- 2014/12/17 06:00
MHDA- 2015/10/22 06:00
CRDT- 2014/12/16 06:00
PHST- 2014/09/09 00:00 [received]
PHST- 2014/11/13 00:00 [revised]
PHST- 2014/12/07 00:00 [accepted]
PHST- 2014/12/16 06:00 [entrez]
PHST- 2014/12/17 06:00 [pubmed]
PHST- 2015/10/22 06:00 [medline]
AID - 10.1111/jne.12245 [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2015 Feb;27(2):111-22. doi: 10.1111/jne.12245.