PMID- 25499200
OWN - NLM
STAT- MEDLINE
DCOM- 20170417
LR  - 20220331
IS  - 1532-2742 (Electronic)
IS  - 0163-4453 (Linking)
VI  - 70
IP  - 6
DP  - 2015 Jun
TI  - Cellular microRNA-miR-548g-3p modulates the replication of dengue virus.
PG  - 631-40
LID - S0163-4453(14)00372-7 [pii]
LID - 10.1016/j.jinf.2014.12.001 [doi]
AB  - OBJECTIVE: It has been well recognized that microRNA plays a role in the 
      host-pathogen interaction network. The significance of microRNA in the regulation 
      of dengue virus (DENV) replication, however, remains unknown. The objective of 
      our study was to determine the biological function of miR-548g-3p in modulating 
      the replication of dengue virus. METHODS: Here we report that employment of a 
      microRNA target search algorithm to analyze the 5' untranslated region (5'UTR) 
      consensus sequences of DENV (DENV serotypes 1-4) led to a discovery that 
      miR-548g-3p directly targets the stem loop A promoter element within the 5'UTR, a 
      region essential for DENV replication. Real-time PCR was used to measure the 
      expression levels of miR-548g-3p under DENV infection. We performed 
      overexpression and inhibition assays to test the role of miR-548g-3p on DENV 
      replication. The protein and mRNA levels of interferon were measured by ELISA and 
      real-time PCR respectively. RESULT: We found that overexpression of miR-548g-3p 
      suppressed multiplication of DENV 1, 2, 3 and 4, and that miR-548g-3p was also 
      found to interfere with DENV translation, thereby suppressing the expression of 
      viral proteins. CONCLUSION: Our results suggest that miR-548g-3p directly 
      regulates DENV replication and warrant further study to investigate the 
      feasibility of microRNA-based anti-DENV approaches.
CI  - Copyright (c) 2014 The British Infection Association. Published by Elsevier Ltd. 
      All rights reserved.
FAU - Wen, Weitao
AU  - Wen W
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou 510080, China; Guangdong Province Key Laboratory of 
      Functional Molecules in Oceanic Microorganism (Sun Yat-sen University), Bureau of 
      Education, Guangzhou 510080, China; Department of Microbiology, Zhongshan School 
      of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
FAU - He, Zhenjian
AU  - He Z
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou 510080, China; Guangdong Province Key Laboratory of 
      Functional Molecules in Oceanic Microorganism (Sun Yat-sen University), Bureau of 
      Education, Guangzhou 510080, China; Department of Microbiology, Zhongshan School 
      of Medicine, Sun Yat-sen University, Guangzhou 510080, China; Department of 
      Medical Statistics and Epidemiology, School of Public Health, Sun Yat-Sen 
      University, Guangzhou 510080, China.
FAU - Jing, Qinlong
AU  - Jing Q
AD  - Department of Medical Statistics and Epidemiology, School of Public Health, Sun 
      Yat-Sen University, Guangzhou 510080, China; Department of Infectious Diseases, 
      Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
FAU - Hu, Yiwen
AU  - Hu Y
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou 510080, China; Guangdong Province Key Laboratory of 
      Functional Molecules in Oceanic Microorganism (Sun Yat-sen University), Bureau of 
      Education, Guangzhou 510080, China; Department of Microbiology, Zhongshan School 
      of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
FAU - Lin, Cuiji
AU  - Lin C
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou 510080, China; Guangdong Province Key Laboratory of 
      Functional Molecules in Oceanic Microorganism (Sun Yat-sen University), Bureau of 
      Education, Guangzhou 510080, China; Department of Microbiology, Zhongshan School 
      of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
FAU - Zhou, Rui
AU  - Zhou R
AD  - Department of Clinical Medicine, Zhongshan School of Medicine, Sun Yat-sen 
      University, Guangzhou 510080, China.
FAU - Wang, Xiaoqun
AU  - Wang X
AD  - Department of Clinical Medicine, Zhongshan School of Medicine, Sun Yat-sen 
      University, Guangzhou 510080, China.
FAU - Su, Yangfan
AU  - Su Y
AD  - Department of Clinical Medicine, Zhongshan School of Medicine, Sun Yat-sen 
      University, Guangzhou 510080, China.
FAU - Yuan, Jiehao
AU  - Yuan J
AD  - Department of Clinical Medicine, Zhongshan School of Medicine, Sun Yat-sen 
      University, Guangzhou 510080, China.
FAU - Chen, Zhenxin
AU  - Chen Z
AD  - Department of Clinical Medicine, Zhongshan School of Medicine, Sun Yat-sen 
      University, Guangzhou 510080, China.
FAU - Yuan, Jie
AU  - Yuan J
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou 510080, China; Guangdong Province Key Laboratory of 
      Functional Molecules in Oceanic Microorganism (Sun Yat-sen University), Bureau of 
      Education, Guangzhou 510080, China; Department of Biochemistry, Zhongshan School 
      of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
FAU - Wu, Jueheng
AU  - Wu J
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou 510080, China; Guangdong Province Key Laboratory of 
      Functional Molecules in Oceanic Microorganism (Sun Yat-sen University), Bureau of 
      Education, Guangzhou 510080, China; Department of Microbiology, Zhongshan School 
      of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
FAU - Li, Jun
AU  - Li J
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou 510080, China; Guangdong Province Key Laboratory of 
      Functional Molecules in Oceanic Microorganism (Sun Yat-sen University), Bureau of 
      Education, Guangzhou 510080, China; Department of Biochemistry, Zhongshan School 
      of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
FAU - Zhu, Xun
AU  - Zhu X
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou 510080, China; Guangdong Province Key Laboratory of 
      Functional Molecules in Oceanic Microorganism (Sun Yat-sen University), Bureau of 
      Education, Guangzhou 510080, China; Department of Microbiology, Zhongshan School 
      of Medicine, Sun Yat-sen University, Guangzhou 510080, China. Electronic address: 
      zhuxun8@mail.sysu.edu.cn.
FAU - Li, Mengfeng
AU  - Li M
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou 510080, China; Guangdong Province Key Laboratory of 
      Functional Molecules in Oceanic Microorganism (Sun Yat-sen University), Bureau of 
      Education, Guangzhou 510080, China; Department of Microbiology, Zhongshan School 
      of Medicine, Sun Yat-sen University, Guangzhou 510080, China. Electronic address: 
      limf@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141211
PL  - England
TA  - J Infect
JT  - The Journal of infection
JID - 7908424
RN  - 0 (5' Untranslated Regions)
RN  - 0 (MIRN548 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Animals
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Consensus Sequence
MH  - Dengue/*virology
MH  - Dengue Virus/*physiology
MH  - Gene Expression
MH  - Genes, Reporter
MH  - *Host-Pathogen Interactions
MH  - Humans
MH  - Interferons/genetics/metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - Protein Biosynthesis
MH  - RNA, Messenger/genetics
MH  - RNA, Viral/genetics
MH  - Vero Cells
MH  - Viral Proteins/genetics/metabolism
MH  - *Virus Replication
OTO - NOTNLM
OT  - Dengue virus
OT  - Viral replication
OT  - miR-548g-3p
OT  - microRNA
EDAT- 2014/12/17 06:00
MHDA- 2017/04/18 06:00
CRDT- 2014/12/16 06:00
PHST- 2014/09/18 00:00 [received]
PHST- 2014/11/30 00:00 [revised]
PHST- 2014/12/06 00:00 [accepted]
PHST- 2014/12/16 06:00 [entrez]
PHST- 2014/12/17 06:00 [pubmed]
PHST- 2017/04/18 06:00 [medline]
AID - S0163-4453(14)00372-7 [pii]
AID - 10.1016/j.jinf.2014.12.001 [doi]
PST - ppublish
SO  - J Infect. 2015 Jun;70(6):631-40. doi: 10.1016/j.jinf.2014.12.001. Epub 2014 Dec 
      11.