PMID- 25499299
OWN - NLM
STAT- MEDLINE
DCOM- 20151023
LR  - 20150216
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 197
DP  - 2015 Feb 2
TI  - Cysteine residues of the porcine reproductive and respiratory syndrome virus 
      ORF5a protein are not essential for virus viability.
PG  - 17-25
LID - S0168-1702(14)00509-7 [pii]
LID - 10.1016/j.virusres.2014.12.004 [doi]
AB  - ORF5a protein was recently identified as a novel structural protein in porcine 
      reproductive and respiratory syndrome virus (PRRSV). The ORF5a protein possesses 
      two cysteines at positions 29 and 30 that are highly conserved among type 2 
      PRRSV. In this study, the significance of the ORF5a protein cysteine residues on 
      virus replication was determined based on a type 2 PRRSV cDNA clone (pAJXM). Each 
      cysteine was substituted by serine or glycine and the mutations were introduced 
      into pAJXM. We found that the replacement of cysteine to glycine at position 30 
      was lethal for virus viability, but all serine mutant clones produced infectious 
      progeny viruses. This data indicated that cysteine residues in the ORF5a protein 
      were not essential for replication of type 2 PRRSV. The bimolecular fluorescence 
      complementation (BiFC) and Co-immunoprecipitation (Co-IP) assay were used to 
      study ORF5a protein interacted with other enveloped proteins. These results 
      showed that ORF5a protein interacted non-covalently with itself and interacted 
      with GP4 and 2b protein. The replacement of cysteine to glycine at position 30 
      affected the ORF5a protein interacted non-covalently with itself, which may 
      account for the lethal phenotype of mutants carrying substitution of cysteine to 
      glycine at position 30.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Sun, Lichang
AU  - Sun L
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China; Key 
      Laboratory of Animal Diseases Diagnostic and Immunology, College of Veterinary 
      Medicine, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 
      210095, PR China.
FAU - Zhou, Yan
AU  - Zhou Y
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Liu, Runxia
AU  - Liu R
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Li, Yanhua
AU  - Li Y
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Gao, Fei
AU  - Gao F
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Wang, Xiaomin
AU  - Wang X
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Fan, Hongjie
AU  - Fan H
AD  - Key Laboratory of Animal Diseases Diagnostic and Immunology, College of 
      Veterinary Medicine, Nanjing Agricultural University, Ministry of Agriculture, 
      Nanjing 210095, PR China.
FAU - Yuan, Shishan
AU  - Yuan S
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Wei, Zuzhang
AU  - Wei Z
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China; College of 
      Animal Science and Technology, Guangxi University, Nanning 530005, PR China. 
      Electronic address: zuzhangwei@163.com.
FAU - Tong, Guangzhi
AU  - Tong G
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. Electronic 
      address: gztong@shvri.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141210
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Amino Acid Substitution
MH  - Animals
MH  - Cell Line
MH  - Conserved Sequence
MH  - Cysteine/genetics/*metabolism
MH  - DNA Mutational Analysis
MH  - *Microbial Viability
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - Swine
MH  - Viral Envelope Proteins/genetics/*metabolism
MH  - Virus Replication
OTO - NOTNLM
OT  - Cysteine
OT  - ORF5a
OT  - PRRSV
OT  - Replication
EDAT- 2014/12/17 06:00
MHDA- 2015/10/24 06:00
CRDT- 2014/12/16 06:00
PHST- 2014/08/01 00:00 [received]
PHST- 2014/11/28 00:00 [revised]
PHST- 2014/12/02 00:00 [accepted]
PHST- 2014/12/16 06:00 [entrez]
PHST- 2014/12/17 06:00 [pubmed]
PHST- 2015/10/24 06:00 [medline]
AID - S0168-1702(14)00509-7 [pii]
AID - 10.1016/j.virusres.2014.12.004 [doi]
PST - ppublish
SO  - Virus Res. 2015 Feb 2;197:17-25. doi: 10.1016/j.virusres.2014.12.004. Epub 2014 
      Dec 10.