PMID- 25501204
OWN - NLM
STAT- MEDLINE
DCOM- 20150810
LR  - 20141216
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 13
IP  - 4
DP  - 2014 Nov 27
TI  - Overexpression of protein kinase B/AKT induces phosphorylation of p70S6K and 
      4E-BP1 in goat fetal fibroblasts.
PG  - 9931-8
LID - 10.4238/2014.November.27.22 [doi]
AB  - Protein kinases regulate many processes, including cell growth, metabolism, 
      molecular interactions, and cell proliferation. Protein kinase B (PKB)/AKT (v-AKT 
      mouse thymoma viral oncogene homolog) is an upstream component of mammalian 
      target of rapamycin (mTOR) signaling and mediates pathophysiological processes in 
      several signaling pathways. This study aimed to construct and overexpress a 
      eukaryotic goat AKT expression vector in goat fetal fibroblasts and examine the 
      effects of AKT on the phosphorylation of p70S6K and 4E-BP1. AKT was subcloned 
      into the expression vector pIRES2-DsRed2 to generate pIRES2-DsRed2-AKT, which was 
      transfected into goat fetal fibroblasts with LipofectamineTM 2000. AKT was 
      measured by reverse transcription-polymerase chain reaction in the transgenic 
      cells, and the expression of AKT and phosphorylation of p70S6K (Thr389) and 
      4E-BP1 (Thr37/46) were analyzed by Western blot. Cell clones that stably emitted 
      red fluorescence were obtained after transfection for 48 h, and the exogenous 
      gene was verified. Exogenous AKT was transcribed, and AKT was overexpressed, 
      inducing the phosphorylation of p70S6K (Thr389) and 4E-BP1 (Thr37/46) in goat 
      fetal fibroblasts. Thus, the overexpression of AKT activates mTOR signaling in 
      goat cells.
FAU - Ma, K L
AU  - Ma KL
AD  - College of Life Sciences, Inner Mongolia University, Hohhot, China.
FAU - Nyamtsengel, V
AU  - Nyamtsengel V
AD  - College of Life Sciences, Inner Mongolia University, Hohhot, China.
FAU - Bao, W L
AU  - Bao WL
AD  - College of Life Sciences, Inner Mongolia University, Hohhot, China.
FAU - Lian, M Y
AU  - Lian MY
AD  - College of Life Sciences, Inner Mongolia University, Hohhot, China.
FAU - Wang, W P
AU  - Wang WP
AD  - College of Life Sciences, Inner Mongolia University, Hohhot, China.
FAU - Wang, Y F
AU  - Wang YF
AD  - College of Life Sciences, Inner Mongolia University, Hohhot, China.
FAU - Wang, X
AU  - Wang X
AD  - College of Life Sciences, Inner Mongolia University, Hohhot, China.
FAU - Wang, Z G
AU  - Wang ZG
AD  - College of Life Sciences, Inner Mongolia University, Hohhot, China 
      lswzg@imu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141127
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (Carrier Proteins)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/*metabolism
MH  - Clone Cells
MH  - Fetus/*cytology
MH  - Fibroblasts/*enzymology
MH  - Genetic Vectors/metabolism
MH  - Goats/*embryology
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Recombination, Genetic/genetics
MH  - Ribosomal Protein S6 Kinases, 70-kDa/*metabolism
MH  - Transfection
MH  - Transgenes
EDAT- 2014/12/17 06:00
MHDA- 2015/08/11 06:00
CRDT- 2014/12/16 06:00
PHST- 2014/12/16 06:00 [entrez]
PHST- 2014/12/17 06:00 [pubmed]
PHST- 2015/08/11 06:00 [medline]
AID - gmr4356 [pii]
AID - 10.4238/2014.November.27.22 [doi]
PST - epublish
SO  - Genet Mol Res. 2014 Nov 27;13(4):9931-8. doi: 10.4238/2014.November.27.22.