PMID- 25501334
OWN - NLM
STAT- MEDLINE
DCOM- 20150818
LR  - 20181113
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 15
IP  - 12
DP  - 2014 Dec 9
TI  - 14-3-3 proteins participate in light signaling through association with 
      PHYTOCHROME INTERACTING FACTORs.
PG  - 22801-14
LID - 10.3390/ijms151222801 [doi]
AB  - 14-3-3 proteins are regulatory proteins found in all eukaryotes and are known to 
      selectively interact with phosphorylated proteins to regulate physiological 
      processes. Through an affinity purification screening, many light-related 
      proteins were recovered as 14-3-3 candidate binding partners. Yeast two-hybrid 
      analysis revealed that the 14-3-3 kappa isoform (14-3-3kappa) could bind to 
      PHYTOCHROME INTERACTING FACTOR3 (PIF3) and CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). 
      Further analysis by in vitro pull-down assay confirmed the interaction between 
      14-3-3kappa and PIF3. Interruption of putative phosphorylation sites on the 14-3-3 
      binding motifs of PIF3 was not sufficient to inhibit 14-3-3kappa from binding or to 
      disturb nuclear localization of PIF3. It was also indicated that 14-3-3kappa could 
      bind to other members of the PIF family, such as PIF1 and PIF6, but not to LONG 
      HYPOCOTYL IN FAR-RED1 (HFR1). 14-3-3 mutants, as well as the PIF3 overexpressor, 
      displayed longer hypocotyls, and a pif3 mutant displayed shorter hypocotyls than 
      the wild-type in red light, suggesting that 14-3-3 proteins are positive 
      regulators of photomorphogenesis and function antagonistically with PIF3. 
      Consequently, our results indicate that 14-3-3 proteins bind to PIFs and initiate 
      photomorphogenesis in response to a light signal.
FAU - Adams, Eri
AU  - Adams E
AD  - RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, 
      Yokohama, Kanagawa 230-0045, Japan. eri.adams@riken.jp.
FAU - Diaz, Celine
AU  - Diaz C
AD  - RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, 
      Yokohama, Kanagawa 230-0045, Japan. celine.diaz@cragenomica.es.
FAU - Hong, Jong-Pil
AU  - Hong JP
AD  - RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, 
      Yokohama, Kanagawa 230-0045, Japan. jongpil.hong@riken.jp.
FAU - Shin, Ryoung
AU  - Shin R
AD  - RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, 
      Yokohama, Kanagawa 230-0045, Japan. ryoung.shin@riken.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141209
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (14-3-3 Proteins)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 11121-56-5 (Phytochrome)
SB  - IM
MH  - 14-3-3 Proteins/genetics/*metabolism
MH  - Arabidopsis/genetics/metabolism/radiation effects
MH  - Basic Helix-Loop-Helix Transcription Factors/*metabolism
MH  - *Light
MH  - Models, Biological
MH  - Mutation
MH  - Phenotype
MH  - Photosynthesis
MH  - Phytochrome/*metabolism
MH  - Protein Binding
MH  - Protein Interaction Mapping
MH  - Protein Transport
MH  - Signal Transduction/*radiation effects
MH  - Two-Hybrid System Techniques
PMC - PMC4284738
EDAT- 2014/12/17 06:00
MHDA- 2015/08/19 06:00
PMCR- 2014/12/01
CRDT- 2014/12/16 06:00
PHST- 2014/10/31 00:00 [received]
PHST- 2014/11/27 00:00 [revised]
PHST- 2014/12/04 00:00 [accepted]
PHST- 2014/12/16 06:00 [entrez]
PHST- 2014/12/17 06:00 [pubmed]
PHST- 2015/08/19 06:00 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - ijms151222801 [pii]
AID - ijms-15-22801 [pii]
AID - 10.3390/ijms151222801 [doi]
PST - epublish
SO  - Int J Mol Sci. 2014 Dec 9;15(12):22801-14. doi: 10.3390/ijms151222801.