PMID- 25501660
OWN - NLM
STAT- MEDLINE
DCOM- 20150604
LR  - 20220317
IS  - 1432-0584 (Electronic)
IS  - 0939-5555 (Linking)
VI  - 94
IP  - 5
DP  - 2015 May
TI  - Comparison and evaluation of three screening tests of hereditary spherocytosis in 
      Chinese patients.
PG  - 747-51
LID - 10.1007/s00277-014-2270-2 [doi]
AB  - The objective of this study is to compare and evaluate the diagnostic value of 
      hereditary spherocytosis (HS) by three screening tests, comparing mean spherical 
      corpuscular volume (MSCV) to mean corpuscular volume (MCV), mean corpuscular 
      hemoglobin concentration (MCHC), and flow cytometric osmotic fragility test. 
      Peripheral blood was collected from 237 participators diagnosed at the First 
      Affiliated Hospital of Guangxi Medical University, including 56 hereditary 
      spherocytosis patients, 86 thalassemia patients, and 95 healthy people. The 
      samples were examined by three tests, and the three screening tests were 
      evaluated by the sensitivity and specificity of tests. The sensitivity was only 
      41.07%, and specificity was 94.47% when using MCHC >355 g/L as diagnostic 
      criteria. The sensitivity was 89.28%, and specificity was 96.14% when using 
      MSCV < MCV as the optimum cutoff point. When using the residual red cell 
      percentage <23.6% as the diagnostic threshold in flow cytometric osmotic 
      fragility test, the sensitivity was 85.71% and the specificity was 97.24%. Flow 
      cytometry osmotic fragility test or comparing MSCV to MCV combined with smear 
      examination of peripheral red blood cells morphology can be a simple, practical, 
      and accurate hereditary spherocytosis (HS) laboratory screening method.
FAU - Tao, Yi-feng
AU  - Tao YF
AD  - Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, China.
FAU - Deng, Zeng-fu
AU  - Deng ZF
FAU - Liao, Lin
AU  - Liao L
FAU - Qiu, Yu-ling
AU  - Qiu YL
FAU - Chen, Wen-qiang
AU  - Chen WQ
FAU - Lin, Fa-quan
AU  - Lin FQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141212
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
RN  - 0 (Ankyrins)
RN  - 0 (Hemoglobins)
RN  - Spherocytosis, Type 1
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Ankyrins/*deficiency
MH  - Child
MH  - Child, Preschool
MH  - Erythrocytes/metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Hemoglobins/metabolism
MH  - Humans
MH  - Male
MH  - Osmotic Fragility/physiology
MH  - Spherocytosis, Hereditary/*diagnosis
MH  - Thalassemia/diagnosis
MH  - Young Adult
EDAT- 2014/12/17 06:00
MHDA- 2015/06/05 06:00
CRDT- 2014/12/16 06:00
PHST- 2014/07/28 00:00 [received]
PHST- 2014/11/27 00:00 [accepted]
PHST- 2014/12/16 06:00 [entrez]
PHST- 2014/12/17 06:00 [pubmed]
PHST- 2015/06/05 06:00 [medline]
AID - 10.1007/s00277-014-2270-2 [doi]
PST - ppublish
SO  - Ann Hematol. 2015 May;94(5):747-51. doi: 10.1007/s00277-014-2270-2. Epub 2014 Dec 
      12.