PMID- 2550815
OWN - NLM
STAT- MEDLINE
DCOM- 19891025
LR  - 20131121
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 3
IP  - 8
DP  - 1989 Aug
TI  - A quantitative comparison of dual control of a hormone response element by 
      progestins and glucocorticoids in the same cell line.
PG  - 1270-8
AB  - Progesterone receptor-containing T47D human breast cancer cells are responsive to 
      progestins but fail to respond to other steroid hormones, in particular 
      dexamethasone, because they have no measurable levels of receptors for estrogens, 
      androgens, or glucocorticoids. To quantitatively study dual responsiveness of the 
      mouse mammary tumor virus (MMTV) promoter to progestins and glucocorticoids, we 
      have stably transfected T47D cells with a glucocorticoid receptor (GR) expression 
      vector. A cloned derivative (A1-2) was isolated that expresses a normal, full 
      length GR, as assessed by steroid binding and Western immunoblot with a 
      monoclonal anti-GR antibody. Moreover, GR is expressed at levels (80,000-100,000 
      molecules per cell) comparable to the high levels of endogenous progesterone 
      receptor (200,000 molecules per cell). In A1-2 cells transiently transfected with 
      an MMTV-chloramphenicol acetyl transferase reporter gene, induction by 
      glucocorticoid was substantially greater (5-fold) than induction mediated by 
      progestins. These results suggest that glucocorticoids may be the primary 
      regulator of MMTV.
FAU - Nordeen, S K
AU  - Nordeen SK
AD  - Department of Pathology, University of Colorado Health Sciences Center, Denver 
      80262.
FAU - Kuhnel, B
AU  - Kuhnel B
FAU - Lawler-Heavner, J
AU  - Lawler-Heavner J
FAU - Barber, D A
AU  - Barber DA
FAU - Edwards, D P
AU  - Edwards DP
LA  - eng
GR  - CA-41386/CA/NCI NIH HHS/United States
GR  - CA-46938/CA/NCI NIH HHS/United States
GR  - DK-37061/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (Glucocorticoids)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Progesterone)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Breast Neoplasms
MH  - Glucocorticoids/*physiology
MH  - Humans
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Plasmids
MH  - Progesterone/physiology
MH  - Promoter Regions, Genetic
MH  - Receptors, Glucocorticoid/*physiology
MH  - Receptors, Progesterone/*physiology
MH  - *Regulatory Sequences, Nucleic Acid
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1989/08/01 00:00
MHDA- 1989/08/01 00:01
CRDT- 1989/08/01 00:00
PHST- 1989/08/01 00:00 [pubmed]
PHST- 1989/08/01 00:01 [medline]
PHST- 1989/08/01 00:00 [entrez]
AID - 10.1210/mend-3-8-1270 [doi]
PST - ppublish
SO  - Mol Endocrinol. 1989 Aug;3(8):1270-8. doi: 10.1210/mend-3-8-1270.