PMID- 25509160
OWN - NLM
STAT- MEDLINE
DCOM- 20150120
LR  - 20161125
IS  - 0041-3771 (Print)
IS  - 0041-3771 (Linking)
VI  - 56
IP  - 4
DP  - 2014
TI  - [Effect of bone marrow cells transplantation on the decidua formation in 
      pseudopregnant rats].
PG  - 268-72
AB  - One of the most common causes of the current pregnancy loss is the failure of the 
      decidual reaction of endometrial cells. It is assumed that a partial source of 
      decidual cells in endometrial tissue is bone marrow cells (BMCs). In the present 
      work, we have studied possible effect of BMCs transplantation on the process of 
      decidualization using the model of pseudopregnancy in rats. BMCs were flushed 
      from the rat femurs and tibias. The obtained suspension of single BMCs was 
      injected into one of rat uterine horns on the 5th day of pseudopregnancy. PBS 
      without cells was injected into the contralateral horn served as the control. 
      Rats were sacrificed on the 11th day of pseudopregnancy. Decidua formed in the 
      experimental uterine horn showed an increase in the meso-antimezometral direction 
      of their diameter of about 1.5-2 times as compared with a control horn. The 
      weight of decidual tissue in the experimental horn exceeded 3 times the weight of 
      the control one. The presence of transplanted BMCs in decidual tissue was 
      documented by preliminary double staining of BMCs with membrane dye PKH 26 Red 
      and nuclear dye Hoechst 33342. Histological analysis of decidua sections after 
      transplantation revealed any alterations neither in cell differentiation nor in 
      tissue structure. We conclude that BMCs transplantation stimulates 
      decidualization in animals.
FAU - Domnina, A P
AU  - Domnina AP
FAU - Mikhailov, V M
AU  - Mikhailov VM
FAU - Nikol'skii, N N
AU  - Nikol'skii NN
LA  - rus
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Russia (Federation)
TA  - Tsitologiia
JT  - Tsitologiia
JID - 0417363
RN  - 0 (Benzimidazoles)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Organic Chemicals)
RN  - 0 (PKH 26)
RN  - P976261J69 (bisbenzimide ethoxide trihydrochloride)
SB  - IM
MH  - Animals
MH  - Benzimidazoles
MH  - Bone Marrow Cells/*cytology
MH  - *Bone Marrow Transplantation
MH  - Cell Proliferation
MH  - Decidua/*cytology/growth & development
MH  - Electric Stimulation
MH  - Female
MH  - Fluorescent Dyes
MH  - Organ Size
MH  - Organic Chemicals
MH  - Pregnancy
MH  - Pseudopregnancy/pathology/*therapy
MH  - Rats
EDAT- 2014/12/17 06:00
MHDA- 2015/01/21 06:00
CRDT- 2014/12/17 06:00
PHST- 2014/12/17 06:00 [entrez]
PHST- 2014/12/17 06:00 [pubmed]
PHST- 2015/01/21 06:00 [medline]
PST - ppublish
SO  - Tsitologiia. 2014;56(4):268-72.