PMID- 25509269
OWN - NLM
STAT- MEDLINE
DCOM- 20150423
LR  - 20161018
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 34
IP  - 10
DP  - 2014 Oct
TI  - [Effect of shenluotong decoction on renal interstitial fibrosis in rats with 
      obstructive nephropathy].
PG  - 1238-44
AB  - OBJECTIVE: To observe the effect of Shenluotong Decoction (SD) on serum levels of 
      aldosterone, monocyte chemoattractant protein-1 (MCP-1), alpha-smooth muscle protein 
      (alpha-SMA), and nuclear factor-KB (NF-kappaB) in obstructive nephropathy rats, and to 
      explore the initial mechanism of SD for inhibiting renal interstitial fibrosis. 
      METHODS: Totally 48 healthy Wistar rats were randomly divided into the 
      sham-operation group (n =12) and the model group (n =36). Renal interstitial 
      fibrosis rat model was established by unilateral ureteral obstruction (UUO). 
      After successful modeling, 36 rats were randomly divided into the model group, 
      the Chinese medicine group, and the Western medicine group, 12 in each group. 
      Eplerenone was added in the forage at the daily dose of 100 mg/kg for rats in the 
      Western medicine group. Chinese medicine was added in the forage at the daily 
      dose of 26 g/kg for rats in the Chinese medicine group. Equal volume of normal 
      saline was administered to rats in the sham-operation group and the model group. 
      All medication was performed once daily. The obstructive kidneys were extracted 
      ten days after medication. The pathomorphological changes were observed. The 
      contents of serum aldosterone and MCP-1, and the protein or mRNA expression of 
      MCP-1, alpha-SMA, and NF-KB were detected. RESULTS: Compared with the sham-operation 
      group, infiltration of a large amount of inflammatory cells and collagen 
      deposition significantly increased, serum contents of aldosterone and MCP-1 
      obviously increased (P < 0.01), the expression of MCP-1 mRNA and protein were 
      significantly up-regulated (P <0.01), the protein expression of alpha-SMA and NF-KB 
      were significantly enhanced in the model group (P <0.01). Com- pared with the 
      model group, infiltration of inflammatory cells and renal collagen deposition 
      were attenua- ted in the Chinese medicine group and the Western medicine group, 
      the serum MCP-1 level were reduced, and the mRNA and protein expression of MCP-1 
      were significantly down-regulated (P <0.01), the protein expression of alpha-SMA and 
      NF-KB were obviously inhibited (P <0. 01). At the same time, serum aldosterone 
      level was reduced in the Chinese medicine group (P <0.01). CONCLUSIONS: 
      inflammatory lesions of the renal tissue could promote the progress of 
      interstitial fibrosis in rats with obstructive nephropathy. SD could attenuate 
      interstitial fibrosis through reducing serum contents of aldosterone and MCP-1, 
      down-regulating MCP-1/ NF-KB, and inhibiting the expression of alpha-SMA.
FAU - Wang, Zheng
AU  - Wang Z
FAU - Liang, Li-juan
AU  - Liang LJ
FAU - Wang, Cong-hui
AU  - Wang CH
FAU - Wang, Rui
AU  - Wang R
FAU - Jiang, Guo-wang
AU  - Jiang GW
FAU - Zhang, Xiao-man
AU  - Zhang XM
FAU - An, Ya-juan
AU  - An YJ
FAU - Xu, Qing-you
AU  - Xu QY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Chemokine CCL2)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/drug effects/metabolism
MH  - Down-Regulation/drug effects
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Fibrosis
MH  - Kidney/drug effects
MH  - Kidney Diseases/*drug therapy/genetics
MH  - NF-kappa B/drug effects/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Rats, Sprague-Dawley
MH  - Ureteral Obstruction/*drug therapy/genetics
EDAT- 2014/12/17 06:00
MHDA- 2015/04/24 06:00
CRDT- 2014/12/17 06:00
PHST- 2014/12/17 06:00 [entrez]
PHST- 2014/12/17 06:00 [pubmed]
PHST- 2015/04/24 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 Oct;34(10):1238-44.