PMID- 25509360
OWN - NLM
STAT- MEDLINE
DCOM- 20150210
LR  - 20231213
IS  - 0026-8984 (Print)
IS  - 0026-8984 (Linking)
VI  - 47
IP  - 5
DP  - 2013 Sep-Oct
TI  - [[Screening of differentially expressed miRNAs related to muscle strain and their 
      target genes].
PG  - 868-75
AB  - Muscle strain is one of the most common muscle injuries seen in the office of a 
      practicing physician. To get a better understanding of this injury, we identified 
      the differentially expressed miRNAs in muscle stem cells collected from injured 
      muscle tissues of mouse. In this study, we downloaded the gene expression 
      microarray (GSE26780) from Gene Expression Omnibus database. The dataset 
      contained a total of 12 samples (murine muscle stem cells), including normal 
      controls and samples collected from tissues at different time points after the 
      injury. Differentially expreesed miRNAs were identified by LIMMA package and 
      target genes of mmu-miR-143 were found by TargetScan. Then, a protein-protein in- 
      teraction (PPI) network was constructed for the products of these target genes by 
      using KUPS. Finally, Cytoscape and its plugins were used to identify and analyze 
      the modules in this network. According to the results, 121, 136 and 148 
      differentially expressed miRNAs were identified in injured samples at each time 
      point, and among them, 60 miRNAs were overlapping between all three groups. The 
      expression values of mmu-miR-143 were most significantly altered over time at 
      36-72 h after the injury. Therefore, 510 target genes of mmu-miR-143 were found 
      and a PPI network for the products of these target genes was constructed. 
      Moreover, two modules were identified in the PPI network. Together with the 
      previous studies, we suppose that proteins in module B, most of which are 
      collagens or integrins, most likely participate in healing of strain injuries 
      through cell adhesion processes.
FAU - Shen, Z
AU  - Shen Z
FAU - Li, D
AU  - Li D
FAU - Zhao, Y
AU  - Zhao Y
FAU - Guo, J
AU  - Guo J
LA  - rus
PT  - English Abstract
PT  - Journal Article
PL  - Russia (Federation)
TA  - Mol Biol (Mosk)
JT  - Molekuliarnaia biologiia
JID - 0105454
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN143 microRNA, mouse)
SB  - IM
MH  - Animals
MH  - Gene Expression Regulation/genetics
MH  - Humans
MH  - Mice
MH  - MicroRNAs/*biosynthesis/genetics
MH  - Protein Biosynthesis
MH  - Sprains and Strains/*genetics/pathology/therapy
MH  - Stem Cells/metabolism/pathology
MH  - *Tissue Array Analysis
MH  - Wounds and Injuries/*genetics/metabolism
EDAT- 2013/09/01 00:00
MHDA- 2015/02/11 06:00
CRDT- 2014/12/17 06:00
PHST- 2014/12/17 06:00 [entrez]
PHST- 2013/09/01 00:00 [pubmed]
PHST- 2015/02/11 06:00 [medline]
PST - ppublish
SO  - Mol Biol (Mosk). 2013 Sep-Oct;47(5):868-75.