PMID- 25511139
OWN - NLM
STAT- MEDLINE
DCOM- 20150213
LR  - 20171116
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 143
IP  - 1
DP  - 2015 Jan
TI  - Cytogenetic and flow cytometry evaluation of Richter syndrome reveals MYC, 
      CDKN2A, IGH alterations with loss of CD52, CD62L and increase of CD71 antigen 
      expression as the most frequent recurrent abnormalities.
PG  - 25-35
LID - 10.1309/AJCPATRQWANW2O3N [doi]
AB  - OBJECTIVES: Richter syndrome (RS) is a transformation of chronic lymphocytic 
      leukemia (CLL) or small lymphocytic lymphoma (SLL) into high-grade lymphoma. 
      There are only limited data on flow cytometry (FCM) and cytogenetics in RS. 
      METHODS: In this study, FCM, classic cytogenetics (CC), and fluorescence in situ 
      hybridization (FISH) were performed in eight RS cases. RESULTS: Most cases of RS 
      were characterized by a loss/decrease of CD52 and CD62L and increased CD71 
      expression. CC identified complex karyotypes, with losses of 9/9p and 17/17p as 
      the most frequent in four of seven cases. Seven RS cases demonstrated MYC 
      abnormalities. Disruptions of CDKN2A and IGH were identified in five of seven and 
      four of seven RS cases, respectively. CONCLUSIONS: Newly diagnosed RS is an 
      oncologic emergency, and a quick diagnostic decision is crucial in clinical 
      practice. Therefore, in patients with CLL/SLL and rapidly enlarging asymmetric 
      lymphadenopathy and/or extranodal tumors, we strongly advise FCM of fine-needle 
      aspiration biopsy (FNAB) material, including CD62L, CD52, and CD71 analysis as 
      well as assessment of karyotype and at least MYC abnormalities by FISH of the 
      same FNAB material. Loss of CD52 expression in RS most likely predicts resistance 
      to alemtuzumab therapy, which is frequently used in CLL.
CI  - Copyright(c) by the American Society for Clinical Pathology.
FAU - Woroniecka, Renata
AU  - Woroniecka R
AD  - From the Cancer Genetics Laboratory and renatawr@coi.waw.pl.
FAU - Rymkiewicz, Grzegorz
AU  - Rymkiewicz G
AD  - Flow Cytometry Laboratory of the Pathology Department and Laboratory Diagnostics, 
      the Maria Sklodowska-Curie Memorial Institute and Cancer Centre, Warszawa, 
      Poland;
FAU - Grygalewicz, Beata
AU  - Grygalewicz B
AD  - From the Cancer Genetics Laboratory and.
FAU - Blachnio, Katarzyna
AU  - Blachnio K
AD  - Flow Cytometry Laboratory of the Pathology Department and Laboratory Diagnostics, 
      the Maria Sklodowska-Curie Memorial Institute and Cancer Centre, Warszawa, 
      Poland;
FAU - Rygier, Jolanta
AU  - Rygier J
AD  - From the Cancer Genetics Laboratory and.
FAU - Jarmuz-Szymczak, Malgorzata
AU  - Jarmuz-Szymczak M
AD  - Department of Hematology and Bone Marrow Transplantation, University of Medical 
      Sciences, Poznan, Poland; and Institute of Human Genetics, Polish Academy of 
      Sciences, Poznan, Poland.
FAU - Ratajczak, Blazej
AU  - Ratajczak B
AD  - Department of Hematology and Bone Marrow Transplantation, University of Medical 
      Sciences, Poznan, Poland; and.
FAU - Pienkowska-Grela, Barbara
AU  - Pienkowska-Grela B
AD  - From the Cancer Genetics Laboratory and.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (CD52 Antigen)
RN  - 0 (CD52 protein, human)
RN  - 0 (CD71 antigen)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (Glycoproteins)
RN  - 0 (MYC protein, human)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (Receptors, Transferrin)
RN  - 126880-86-2 (L-Selectin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD/metabolism
MH  - Antigens, Neoplasm/metabolism
MH  - CD52 Antigen
MH  - Cyclin-Dependent Kinase Inhibitor p16/genetics/metabolism
MH  - Cytogenetic Analysis
MH  - Female
MH  - *Flow Cytometry
MH  - Genetic Testing/methods
MH  - Glycoproteins/deficiency/metabolism
MH  - Humans
MH  - L-Selectin/immunology
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-myc/metabolism
MH  - Receptors, Transferrin/metabolism
OTO - NOTNLM
OT  - Alemtuzumab in CLL
OT  - CD52, CD62L, CD71
OT  - Flow cytometry
OT  - MYC, CDKN2A, IGH
OT  - Richter syndrome
EDAT- 2014/12/17 06:00
MHDA- 2015/02/14 06:00
CRDT- 2014/12/17 06:00
PHST- 2014/12/17 06:00 [entrez]
PHST- 2014/12/17 06:00 [pubmed]
PHST- 2015/02/14 06:00 [medline]
AID - 143/1/25 [pii]
AID - 10.1309/AJCPATRQWANW2O3N [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2015 Jan;143(1):25-35. doi: 10.1309/AJCPATRQWANW2O3N.