PMID- 25511566
OWN - NLM
STAT- MEDLINE
DCOM- 20160203
LR  - 20231213
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 23
IP  - 2
DP  - 2015 Jun
TI  - Comparative cytogenetic characterization of primary canine melanocytic lesions 
      using array CGH and fluorescence in situ hybridization.
PG  - 171-86
LID - 10.1007/s10577-014-9444-6 [doi]
AB  - Melanocytic lesions originating from the oral mucosa or cutaneous epithelium are 
      common in the general dog population, with up to 100,000 diagnoses each year in 
      the USA. Oral melanoma is the most frequent canine neoplasm of the oral cavity, 
      exhibiting a highly aggressive course. Cutaneous melanocytomas occur frequently, 
      but rarely develop into a malignant form. Despite the differential prognosis, it 
      has been assumed that subtypes of melanocytic lesions represent the same disease. 
      To address the relative paucity of information about their genomic status, 
      molecular cytogenetic analysis was performed on the three recognized subtypes of 
      canine melanocytic lesions. Using array comparative genomic hybridization (aCGH) 
      analysis, highly aberrant distinct copy number status across the tumor genome for 
      both of the malignant melanoma subtypes was revealed. The most frequent 
      aberrations included gain of dog chromosome (CFA) 13 and 17 and loss of CFA 22. 
      Melanocytomas possessed fewer genome wide aberrations, yet showed a recurrent 
      gain of CFA 20q15.3-17. A distinctive copy number profile, evident only in oral 
      melanomas, displayed a sigmoidal pattern of copy number loss followed immediately 
      by a gain, around CFA 30q14. Moreover, when assessed by fluorescence in situ 
      hybridization (FISH), copy number aberrations of targeted genes, such as gain of 
      c-MYC (80 % of cases) and loss of CDKN2A (68 % of cases), were observed. This 
      study suggests that in concordance with what is known for human melanomas, canine 
      melanomas of the oral mucosa and cutaneous epithelium are discrete and initiated 
      by different molecular pathways.
FAU - Poorman, Kelsey
AU  - Poorman K
AD  - Department of Molecular Biomedical Science, College of Veterinary Medicine, North 
      Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, USA.
FAU - Borst, Luke
AU  - Borst L
FAU - Moroff, Scott
AU  - Moroff S
FAU - Roy, Siddharth
AU  - Roy S
FAU - Labelle, Philippe
AU  - Labelle P
FAU - Motsinger-Reif, Alison
AU  - Motsinger-Reif A
FAU - Breen, Matthew
AU  - Breen M
LA  - eng
GR  - R01 GM098856/GM/NIGMS NIH HHS/United States
GR  - T32 GM081057/GM/NIGMS NIH HHS/United States
GR  - T32GM081057/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20141216
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
SB  - IM
MH  - Animals
MH  - *Chromosome Aberrations
MH  - Cluster Analysis
MH  - *Comparative Genomic Hybridization
MH  - Computational Biology
MH  - DNA Copy Number Variations
MH  - Dogs
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanoma/*genetics/metabolism/pathology
MH  - Mouth Neoplasms/genetics/pathology
MH  - Penetrance
MH  - Skin Neoplasms
MH  - Melanoma, Cutaneous Malignant
PMC - PMC5462112
MID - NIHMS857346
EDAT- 2014/12/17 06:00
MHDA- 2016/02/04 06:00
PMCR- 2017/06/07
CRDT- 2014/12/17 06:00
PHST- 2014/08/26 00:00 [received]
PHST- 2014/10/14 00:00 [accepted]
PHST- 2014/10/07 00:00 [revised]
PHST- 2014/12/17 06:00 [entrez]
PHST- 2014/12/17 06:00 [pubmed]
PHST- 2016/02/04 06:00 [medline]
PHST- 2017/06/07 00:00 [pmc-release]
AID - 10.1007/s10577-014-9444-6 [doi]
PST - ppublish
SO  - Chromosome Res. 2015 Jun;23(2):171-86. doi: 10.1007/s10577-014-9444-6. Epub 2014 
      Dec 16.