PMID- 25515035
OWN - NLM
STAT- MEDLINE
DCOM- 20150928
LR  - 20171116
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 11
IP  - 4
DP  - 2015 Apr
TI  - Gypenosides attenuate cholesterol-induced DNA damage by inhibiting the production 
      of reactive oxygen species in human umbilical vein endothelial cells.
PG  - 2845-51
LID - 10.3892/mmr.2014.3095 [doi]
AB  - Previous studies have demonstrated that DNA damage induces atherosclerosis and 
      that oxidative stress has an important role in DNA damage. Gypenosides (Gps), the 
      main ingredient of Gynostemma Pentaphylla (Thunb.) Makino, have been recognized 
      as specific antioxidants and have previously been reported to inhibit high‑fat 
      diet‑induced atherosclerosis in rats. However, whether or not Gps attenuate DNA 
      damage through their antioxidant effects remains to be elucidated. The current 
      study was performed to clarify whether or not Gps can inhibit cholesterol‑induced 
      DNA damage through antioxidation. The present study provided new insights into 
      the pharmacological effects of Gps on atherosclerosis. HUVECs were treated with 
      Gps at various concentrations (1, 10 and 100 microg/ml) for 1 h. The protective 
      effects of Gps on cholesterol‑induced DNA damage were determined using 
      immunofluorescence, western blotting, reverse‑transcription quantitative 
      polymerase chain reaction and flow cytometry. Pretreatment with Gps (1, 10 and 
      100 microg/ml) effectively attenuated cholesterol‑induced DNA damage in HUVECs by 
      inhibiting phosphorylation of H2AX, a member of the histone family. Furthermore, 
      Gps (100 microg/ml) pretreatment inhibited cholesterol‑induced transcription and 
      activity of nicotinamide adenine dinucleotide phosphate‑oxidase 4 and reduced 
      intracellular ROS levels. In conclusion, Gps attenuated cholesterol‑induced DNA 
      damage by inhibiting ROS production in HUVECs, suggesting that the inhibitory 
      effect of Gps on atherogenesis is correlated with the alleviation of DNA damage.
FAU - Quan, Yuan
AU  - Quan Y
AD  - Department of Biochemistry, Zunyi Medical College, Zunyi, Guizhou 563000, P.R. 
      China.
FAU - Yang, Yijun
AU  - Yang Y
AD  - Department of Biochemistry, Zunyi Medical College, Zunyi, Guizhou 563000, P.R. 
      China.
FAU - Wang, Huixing
AU  - Wang H
AD  - Department of Biochemistry, Zunyi Medical College, Zunyi, Guizhou 563000, P.R. 
      China.
FAU - Shu, Bo
AU  - Shu B
AD  - Department of Biochemistry, Zunyi Medical College, Zunyi, Guizhou 563000, P.R. 
      China.
FAU - Gong, Qi-Hai
AU  - Gong QH
AD  - Department of Pharmacology, Zunyi Medical College, Zunyi, Guizhou 563000, P.R. 
      China.
FAU - Qian, Minzhang
AU  - Qian M
AD  - Department of Biochemistry, Zunyi Medical College, Zunyi, Guizhou 563000, P.R. 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141216
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Antioxidants)
RN  - 0 (Histones)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (gypenoside)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.17.3.2 (Xanthine Oxidase)
RN  - EC 1.6.3.- (NADPH Oxidases)
SB  - IM
MH  - Antioxidants/chemistry/pharmacology
MH  - Cholesterol/*pharmacology
MH  - DNA Damage/*drug effects
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Gynostemma/chemistry
MH  - Histones/metabolism
MH  - Human Umbilical Vein Endothelial Cells/*drug effects/*metabolism
MH  - Humans
MH  - NADPH Oxidases/genetics/metabolism
MH  - Nitric Oxide Synthase/metabolism
MH  - Oxidative Stress
MH  - Plant Extracts/chemistry/pharmacology
MH  - Reactive Oxygen Species/*metabolism
MH  - Xanthine Oxidase/metabolism
EDAT- 2014/12/18 06:00
MHDA- 2015/09/29 06:00
CRDT- 2014/12/18 06:00
PHST- 2013/12/03 00:00 [received]
PHST- 2014/09/12 00:00 [accepted]
PHST- 2014/12/18 06:00 [entrez]
PHST- 2014/12/18 06:00 [pubmed]
PHST- 2015/09/29 06:00 [medline]
AID - 10.3892/mmr.2014.3095 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Apr;11(4):2845-51. doi: 10.3892/mmr.2014.3095. Epub 2014 Dec 
      16.